SUMMARYThe currently known distribution range of Achatina fulica Bowdich, 1822, in the state of São Paulo, Brazil, is presented. The record of A. fulica naturally infested with Aelurostrongylus abstrusus larvae (Railliet, 1898) (Nematoda: Metastrongylidae) can be found in the city of Guaratinguetá. It was found A. fulica with Metastrongylidae larvae without known medical and veterinary importance in the cities of Carapicuíba, Embu-Guaçu, Itapevi, São Caetano do Sul, São Paulo and Taboão da Serra.

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DNA barcoding and morphological characters were used to identify adult snails belonging to the genus Biomphalaria from 17 municipalities in the state of São Paulo, Brazil. The DNA barcode analysis also included twenty-nine sequences retrieved from GenBank. The final data set of 104 sequences of the mitochondrial cytochrome oxidase I (COI) gene was analyzed for K2P intraspecific and interspecific divergences, through tree-reconstruction methods (Neighbor-Joining, Maximum Likelihood and Bayesian inference), and by applying different models (ABGD, bPTP, GMYC) to partition the sequences according to the pattern of genetic variation. Twenty-seven morphological parameters of internal organs were used to identify specimens. The molecular taxonomy of Biomphalaria agreed with the morphological identification of specimens from the same collection locality. DNA barcoding may therefore be a useful supporting tool for identifying Biomphalaria snails in areas at risk for schistosomiasis.

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We evaluated the potential of metabarcoding in assessing the environmental DNA (eDNA) biodiversity profile in the water column of an hydroelectric power plant reservoir in southeast Brazil. Samples were obtained in three technical replicates at 1 km from the dam at 1, 13 and 25 m depths. For each minibarcodes -- COI, 12S and 16S -- 1.5 million paired-reads (150 base pairs) were sequenced. A total of 44 unique taxa were found. COI identified most of the taxa (34 taxa; 77.2 %) followed by 16S (14; 31.8 %) and 12S (10; 22.7 %). All minibarcodes identified fishes (13 taxa), however, COI detected other aquatic macro-invertebrates (18), algae (3) and amoebas (2). Richness was the same across the three depths (35 taxa), although, beta diversity suggested slightly divergent profiles. In just one location we identified 15 taxa never reported previously, 50% of the fish species identified in the last year of fishery monitoring and 13% of the species in biodiversity surveys performed from 2012 to 2021. Clustering into Amplicon Sequence Variants (ASV) showed that 12S and 16S are able to detect predominant haplotypes of fishes, suggesting they are suitable to study population genetics of this group. In this study we reviewed the species occurring within the Três Irmãos reservoir according to previous conventional surveys and demonstrated that eDNA metabarcoding can be applied to monitor its biodiversity.

e descrever a morfologia das larvas emergidas. MATERIAIS E MÉTODOS: Moluscos foram coletados em 53 valas. No laboratório, procedeu-se à identificação morfológica dos espécimes e pesquisas parasitológicas para determinação e descrição morfológica das larvas. RESULTADOS: Foram coletados 5.969 moluscos pertencentes às famílias Planorbidae, Lymnaeidae, Physidae, Thiaridae e Ampullariidae. Os exames parasitológicos revelaram nove formas larvais correspondentes a sete morfotipos: xifidiocercária, equinocercária, estrigeocercária, distoma brevifurcada faringeada, distoma brevifurcada afaringeada, pleurolofocercária e anfistomocercária. A espécie Biomphalaria tenagophila (d'Orbigny, 1835) foi suscetível a oito cercárias das nove encontradas; e seis exemplares de moluscos estavam parasitados por larvas de Schistosoma mansoni Sambon, 1907. CONCLUSÃO: É fundamental estabelecer programas de controle e vigilância malacológica em áreas vulneráveis quando moluscos hospedeiros intermediários de parasitas de importância médica e veterinária colonizam esses ambientes, sendo especialmente importante quando esses moluscos estão infectados com larvas de S. mansoni.