Pattern of Genetic Divergence of Mitochondrial DNA Sequences in Biomphalaria tenagophila Complex Species Based on Barcode and Morphological Analysis

Tuan, Roseli; Ohlweiler, Fernanda Pires; Palasio, Raquel Gardini Sanches; Zanna, Ricardo Dalla; Guimarães, Marisa Cristina de Almeida

doi:10.5772/25550

Cited by 16 publications

(15 citation statements)

References 37 publications

(12 reference statements)

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“…The purified genomic DNA then underwent amplification of the mitochondrial cytochrome oxidase I gene using LCO-1490 and HCO-2198 primers (Folmer 1994), according to DNA Barcode protocol (Hebert et al 2003) and PCR conditions described by Tuan et al 2012.…”

Section: Morphological and Molecular Identification Of The Snailsmentioning

confidence: 99%

“…Results obtained for species of freshwater and land mollusks which are medically relevant in the neotropics show the importance of combining traditional methods of morphological analysis with molecular methods to identify known species as well as potential cryptic species (Langand et al 1999, Campbell et al 2000, Dejong et al 2003, Wethington et al 2007, Teodoro et al 2010, Tuan et al 2012, Palasio et al 2017, Bezerra et al 2018.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Biomphalaria spp. freshwater snails and associated mollusks in areas with schistosomiasis risk, using molecular and spatial analysis tools

et al. 2019

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The Middle Paranapanema River region of São Paulo, Brazil is home to significant diversity of Biomphalaria species and is very vulnerable to health and environmental impacts such as schistosomiasis. This study updates freshwater malacological surveys for ecosystems in one portion of the Middle Paranapanema River Basin, with emphasis on the genus Biomphalaria. Snails were collected from 114 distinct bodies of water between 2015 and 2018. Biomphalaria specimens were identified according to morphological and molecular characteristics, while animals in other genera (Drepanotrema, Lymnaea, Melanoides, Physa and Pomacea) were identified solely according to shell characteristics. A geographic information system was used to update intermediate host colonization sites and consequently assist in identifying probable hotspots for intermediate hosts of schistosomiasis. The sequences of the COI gene relating to the DNA barcode stretch were tested for similarity against sequences found in GenBank, for monophyly through Maximum Likelihood phylogenetic inference, and analyzed in ABDG, bPTP and GMYC for the delimitation of putative species. Of the 10,722 snails collected, 86.7% were in the Planorbidae family (75.5% Biomphalaria and 11.2% Drepanotrema) and 13.3% were other non-Planorbidae species (Lymnaea, Melanoides, Physa and Pomacea). The taxonomic COI reference sequences in the NCBI nucleotide database used for DNA sequence comparison, and phylogenetic analysis used to test the monophyly of the groups, resulted in more reliable taxonomic units than delimitation of the COI sequences in MOTUs using statistical taxonomic models. Analysis of the species distribution shows that B. glabrata and B. tenagophila are heterogeneously distributed in the study area. B. glabrata colonizes only five water bodies, in the study area, most of them in Ourinhos, while B. tenagophila predominates in water bodies in Ipaussu. Contrasting with this, B. straminea, B. occidentalis and B. peregrina are evenly distributed throughout the study area.

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Section: Morphological and Molecular Identification Of The Snailsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diversity of Biomphalaria spp. freshwater snails and associated mollusks in areas with schistosomiasis risk, using molecular and spatial analysis tools

et al. 2019

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“…There is evidence of partition of genetic variation in the genus Biomphalaria in different mitochondrial and nuclear haplotypes on a fine geographical scale, indicating that even geographically close populations are genetically differentiated (Mulvey & vRijenhoeK 1982, lanGand et al 1999, MaváReZ et al 2002, tuan & SantoS 2007, tuan et al 2012. The structuring of the genetic variability of Biomphalaria populations on a fine geographical scale may be a result of reproductive characteristics and physiological adaptations that allow the snails to survive droughts and floods through desiccation by reducing their metabolic activity (baRboZa et al 1985, vianey-liaud & lancaStRe 1986, tuan & SiMõeS 1989, baRboSa et al 2012, WeiR & Salice 2012.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the freshwater snail Biomphalaria tenagophila (d’Orbigny, 1835) (Gastropoda: Hygrophila: Planorbidae) across two coastal areas of southeast Brazil

Palasio¹,

Zanotti-Magalhães²,

Tuan³

2018

Folia Malacol.

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This study uses two mitochondrial (COI, 16SrRNA) and one nuclear marker (ITS2) to explore genetic diversity between populations of B. tenagophila (d'Orbigny), the most common intermediate host for Schistosoma mansoni in southeast Brazil. The snails were collected in freshwater bodies along the southern coastal region of Ribeira do Iguape river basin and along the northern coastal region of the state of São Paulo, Brazil. The estimates of genetic differentiation between the populations (Φ) indicate that there is no genetic isolation between northern and southern populations. The haplotype diversity observed in B. tenagophila shows a complex pattern of distribution: in the southern region, the genetic variation in B. tenagophila is partitioned into small and more heterogeneous mitochondrial haplotypes, whereas in the northern region there were one to three homogeneous haplotypes. The results may suggest a different colonisation history across the study area.

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“…Estudos sobre os polimorfismos genéticos vêm sendo utilizados de modo crescente em caramujos do gênero Biomphalaria (LANGAND et al, 1999;VIDIGAL et al, 2000VIDIGAL et al, , 2004ab, 2013CAMPBELL et al, 2000;DEJONG et al, 2003;WETHINGTON et al, 2007;TUAN e SANTOS 2007;TUAN et al, 2012;STANDLEY et al, 2014;PALASIO et al, 2017PALASIO et al, , 2018 VIDIGAL et al, 1994;VERNON et al, 1995;KNIGHT et al, 1999;LANGAND et al, 1999) e técnica do polimorfismo de comprimento dos fragmentos de restrição (RFLP) (KNIGHT et al, 1998).…”

Section: Marcadores Moleculares Aplicados Ao Estudo De Biomphalariaunclassified

“…Também estão relatados na literatura estudos de Biomphalaria com marcadores mitocôndriais: 16S rRNA e Citocromo C Oxidase I (COI) (CAMPBELL et al, 2000;DEJONG et al, 2001;POINTIER et al, 2005;STANDLEY et al, 2014;TUAN et al, 2012;PALASIO et al, 2017PALASIO et al, , 2018); e polimorfismos de nucleotídeo único (SNPs) (KNIGHT et al, 2009;TENNESSEN et al, 2015). A molécula completa de DNA mitocondrial foi sequenciada em B. Estudos têm demonstrado a importância da análise de polimorfismos moleculares mitocondriais e nucleares na identificação espécie-específica e na caracterização de linhagens dentro de uma mesma espécie, os quais não são facilmente diagnosticadas por diferenças morfológicas TUAN e BORTOLATO, 2001;DEJONG et al 2003;WETHINGTON et al 2007;TUAN e SANTOS, 2007;TUAN et al, 2012;PALASIO et al, 2017). A premissa do DNA Barcode é que a variação genética entre espécies diferentes exceda a variação genética dentro de uma mesma espécie (RATNASINGHAM e HEBERT, 2007;CASIRAGHI et al, 2010).…”

Section: Marcadores Moleculares Aplicados Ao Estudo De Biomphalariaunclassified

Padrão de distribuição da diversidade genética molecular e espacial de Biomphalaria spp. e sua relação com a ocorrência da esquistossomose na região do Médio Paranapanema, estado de São Paulo

Palasio¹

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Pattern of Genetic Divergence of Mitochondrial DNA Sequences in Biomphalaria tenagophila Complex Species Based on Barcode and Morphological Analysis

Cited by 16 publications

References 37 publications

Diversity of Biomphalaria spp. freshwater snails and associated mollusks in areas with schistosomiasis risk, using molecular and spatial analysis tools

Diversity of Biomphalaria spp. freshwater snails and associated mollusks in areas with schistosomiasis risk, using molecular and spatial analysis tools

Genetic diversity of the freshwater snail Biomphalaria tenagophila (d’Orbigny, 1835) (Gastropoda: Hygrophila: Planorbidae) across two coastal areas of southeast Brazil

Padrão de distribuição da diversidade genética molecular e espacial de Biomphalaria spp. e sua relação com a ocorrência da esquistossomose na região do Médio Paranapanema, estado de São Paulo

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