There is presented the structure of acute intestinal infections (AIIs) at the outpatient stage of medical care in 135 outpatients (70 men and 65 women, aged from 15 to 55 years) visited the Health Center of Barnaul. In all patients, the mild course of gastroenteritis predominated (the stool frequency did not exceed 4-5 times a day, body temperature - 37.2 0C). The investigation of biological material from patients (feces) was carried out by polymerase chain reaction (PCR) with hybridization-fluorescent detection “AmpliSens® AII screen-FL”. The results showed the high efficiency of the test system used, as in 118 out of 135 samples (87%) there was found genetic material of different etiology, 66.1% were of the viral origin, among which rotaviruses and noroviruses prevailed, 25.4% of samples had bacterial origin (Salmonella prevailed) and 19.5% of mixed virus-viral or bacterial-viral etiology. The work showed both the high sensitivity and specificity of the PCR method in the etiological diagnosis of AII. Among the examined patients, AII of viral origin prevailed (66.1%).
Информация об авторах Мусин Ильдар Наилевич, к.т.н., заведующий кафедрой медицинской инженерии Казанского национального исследовательского технологического университета, г. Казань. 420015, г. Казань, ул. К.Маркса, 68. Тел.: (843) 2314383.
Identification of factors behind the level and duration of persistence of the SARS-CoV-2 antibodies in the blood is assumed to set the direction for studying humoral immunity mechanisms against COVID-19, optimizing the strategy for vaccine use, antibody-based drugs, and epidemiological control of COVID-19. Objective: This study aimed to study the relationship between clinical and demographic characteristics and the level of IgG antibodies to the RBD of SARS-CoV-2 spike protein after COVID-19 in the long term. Residents of the Altai Region of Western Siberia of Russia, Caucasians, aged from 27 to 93 years (median 53.0 years), who recovered from COVID-19 between May 2020 and February 2021 (n = 44) took part in this prospective observational study. The titer of IgG antibodies to the RBD of SARS-CoV-2 spike protein was measured repeatedly in the blood at 4–13 months from the beginning of the clinical manifestation of COVID-19 via the method of enzyme-linked immunosorbent assay. The antibody titer positively correlated with age (p = 0.013) and COVID-19 pneumonia (p = 0.002) at 20–40 and 20–24 weeks from the onset of COVID-19 symptoms, respectively. Age was positively associated with antibody titer regardless of history of COVID-19 pneumonia (beta regression coefficient p = 0.009). The antibody titer decreased in 15 (34.1%) patients, increased in 10 (22.7%) patients, and did not change in 19 (43.2%) patients from the baseline to 48–49 weeks from the onset of COVID-19 symptoms, with seropositivity persisting in all patients. Age and COVID-19 pneumonia are possibly associated with higher IgG antibodies to the spike protein RBD of SARS-CoV-2 following COVID-19 in the long term. Divergent trends of anti-RBD IgG levels in adults illustrate inter-individual differences at 4–13 months from the onset of COVID-19 symptoms.
Highlights Opisthorchiasis is the main helminthiasis of Western Siberia. The clinical picture of opisthorchiasis is non-specific. One of the key signs of opisthorchiasis is hypereosinophilia.
Clinical and laboratory examination of 80 patients with hemophilia aged 16-59 living in the Altai Krai was carried out. From childhood, all patients have repeatedly received cryoprecipitate or fresh frozen plasma. In 50 of 80 patients with hemophilia (62.5%), chronic viral hepatitis (CVH) was diagnosed: CVH B and C in 1 patient (2%), CVH B in 2 (4%) and CVH C in 47 patients (94%). The mean age of patients with hemophilia and CVH is 38.4 (22-59 years). All patients have no data on the prior acute hepatitis B or C. In 30%, we noted an increase of ALT (in 30%), AST (in 20.4%), GGT (in 26.6%), ALP (in 13.3%). The obtained biochemical data indicate the presence of a minimal degree of activity of chronic hepatitis.
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