A solução do picro-sirius foi usada para corar o Paracoccidioides brasiliensis em tecidos humanos fixados em formol. À luz comum, o fungo mostra uma faixa periférica avermelhada e, à luz polarizada, apresenta um ou mais anéis birrefringentes esverdeados. A imersão prévia em solução de hidróxido de sódio a 1%, por alguns minutos, melhora a coloração, sendo o fungo visualizado com mais nitidez à luz polarizada. Por outro lado, a diferenciação dos cortes corados, com picro-sirius em hidróxido de sódio a 0,5%, elimina a birrefringência do colágeno mais rapidamente que a do Paracoccidioides.
The adrenal glands and the central nervous system were studied from five human cases of rabies who died three to six days after first rings of clinical manifestations were seen. In all cases there were Negri bodies in the cytoplasm of neurons of the central nervous system and mononuclear inflammatory cells around blood vessels, more intense in the cases with longer survival. Only the adrenal medulla showed diffuse and intense mononuclear exudate associated with pheocromocytes alterations in 60% of patients. Eosinophilic bodies were found in the cytoplasm of pheochromocytes and in the interstitial space. These bodies may be similar to Negri bodies but their true nature it is not known. The pathogenesis of adrenal medullitis in rabies may be related to embryological and metabolical relationships of the chromaffin system and the nervous system.
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