As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in manyin vitroandin vivostudies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.

This paper analyzes mechanisms of fluorapatite mechanochemical synthesis and its structural characteristics. Several studies of Jokanovic et al. published in appropriate journals and the book “Nanomedicine, the biggest challenge of the 21st century” are the base for this article. Characteristics of obtained materials show numerous biological advantages associated with the specific structural design of material during the process of synthesis. X-ray diffraction (XRD) and infrared spectroscopy with Fourier transform (FTIR) were used for studying the processes of fluorapatite synthesis.

SummaryBackground/Aim: The aim of this study was to evaluate the efficacy of new rotary NiTi instrument XP- endo SHAPER (XPS) used with two irrigation protocols on the root canal cleaning in the apical area.Material and Methods: The research was conducted on 30 single-rooted teeth extracted for orthodontic reasons which were divided into the two groups. Instrumentation of the canals was conducted with XPS instrument and 2% solution of NaOCl was used as irrigant. Instrumentation in the first group was performed using a conventional continuous irrigation, in the second group, protocol of final irrigation was performed intermittently in 3 cycles. The SEM analysis of the apical third of the canal was performed on longitudinal root cross-section standardized photomicrography with a magnification of 2000X.Results: Results showed that a thicker smear layer was observed in the first group and with continuous irrigation protocol (2,10) in relation to the intermittent irrigation protocol in 3 cycles (1,96), but without significant differences.The walls of the root canal in the apical third of the samples of the second group were slightly cleaner (73.3%) in comparison with the teeth of the first group (64, 7%), but also without significant differences.Conclusions: The use of XPS and 2% solution of NaOCl in the root canal enables efficient cleaning of the apical third of tooth. The final irrigation protocol in three cycles improves the efficiency of the smear layer removal in the apical segment of the canal.

Summary Introduction Efficient cleaning and canal design present a basic precondition for successful endodontic treatment. The aim of this study was to evaluate, using SEM analysis, the efficacy of cleaning the apical third of the canal with one instrument in both reciprocating motion and full rotation systems. Materials and methods Thirty (30) single-rooted teeth (upper incisors), extracted due to periodontal reasons, were used and selected in 2 groups. In the group 1, instrumentation was done with a single instrument UNICONE with reciprocal movements (MEDIN, Czech Republic), while in the group 2, a single instrument XPS endo SHAPER (XPS) (FKG, Dentaire, Swiss) with full rotation was used. 2% solution of NaOCl and 17% EDTA solution were used as irrigation agents in both groups. SEM analysis of smear layer in the apical third was done at standard magnification of 1000x and presented on a scale of 1-5. Results showed mostly clean canal walls and no smear layer in the apical region in both tested groups. Canal walls were somewhat cleaner after using UNICONE with reciprocal movements (80%) compared to the canals instrumented with XPS ENDO SHAPER (76%), but this difference was not statistically significant. Conclusion Single-file NiTi systems did not remove smear layer completely, but provided efficient cleaning in the apical part of the canal. UNICONE file with reciprocal movements was somewhat more efficient in removing smear layer compared to the full rotation XPS ENDO SHAPER file.

Introduction. Direct pulp capping procedure is a therapeutic application of a drug on exposed tooth pulp in order to ensure the closure of the pulp chamber and to allow the healing process to take place. Objective. The aim of this study was to examine the histological effects of Emdogain? on exposed tooth pulp of a Vietnamese pig (Sus scrofa verus). Methods. The study comprised 20 teeth of a Vietnamese pig. After class V preparation on the buccal surfaces of incisors, canines and first premolars, pulp was exposed. In the experimental group, the perforations were capped with Emdogain? (Straumann, Basel, Switzerland), while in the control group pulp capping was performed with MTA? (Dentsply Tulsa Dental, Johnson City, TN, USA). All cavities were restored with glass-ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). The observational period was 28 days, after which the animal was sacrificed and histological preparations were made. A light microscope was used to analyze dentin bridge formation, tissue reorganization and inflammation, and the presence of bacteria in the pulp. Results. The formation of dentin bridge was observed in the experimental and control groups. Inflammation of the pulp was mild to moderate in both groups. Angiogenesis and many odontoblast-like cells, responsible for dentin bridge formation, were observed. Necrosis was not observed in any case, nor were bacteria present in the pulp. Conclusion. Histological analysis indicated a favorable therapeutic effect of Emdogain? Gel in direct pulp capping of Vietnamese pigs. Pulp reaction was similar to that of MTA?. [Projekat Ministarstva nauke Republike Srbije, br. ON172026]