The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0.05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.
An increase of reactive oxygen species in the placenta and oxidative disbalance has been recognized as a significant factor contributing to pregnancy complications. Dietary intake of food rich in antioxidants during pregnancy could exert a protective role in the prevention of adverse outcomes such as preeclampsia, miscarriage, and others. Flavonoid taxifolin has shown numerous health-promoting effects in a large number of studies conducted on animals, as well as various human cell types in vitro. However, its effects on human placental cells—trophoblasts—have yet to be determined. Therefore, cytoprotective and genoprotective effects of taxifolin on trophoblast cell line HTR-8/SVneo under induced oxidative stress were explored in this study. Cytotoxicity of a range of taxifolin concentrations (1–150 µM) was evaluated using the MTT and crystal violet assays. A model of oxidative stress was achieved by exposing HTR-8/SVneo cells to H2O2. To determine cytoprotective and antigenotoxic effects, the cells were pre-incubated with three concentrations of taxifolin (10, 50, and 100 µM) and then exposed to H2O2. Taxifolin in concentrations of 1, 5, 10, 25, 50, and 100 µM showed no cytotoxic effects on HTR-8/SVneo cells, but 150 µM of taxifolin caused a significant decrease in adherent cell number, as detected by crystal violet assay. Pretreatment with the chosen concentrations of taxifolin showed a significant cytoprotective effect on H2O2-induced cytotoxicity, as determined by the MTT assay. Furthermore, taxifolin showed a significant reduction in H2O2-induced DNA damage, measured by comet assay. This study showed protective effects of taxifolin on human trophoblast cells exposed to oxidative damage. Further studies are needed to explore the underlying mechanisms.
Oxidative stress and inflammation are DNA instability factors for rheumatoid
arthritis (RA) patients. The aims of this study were to evaluate cytogenetic
alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA
patients: the early and the long-term RA group; and to examine potential of
concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract
(DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment.
A total of 32 RA patients and 10healthy individuals were included. RA
patients were equally divided into four groups: two groups with early phase
RA (one treated with MTX alone, the other in combination with DOLE); and two
long-term phase RA groups (group with active disease and group with low
disease activity)-both treated with MTX and DOLE combination. PBL cultures
were screened for chromosome aberrations and micronuclei frequencies.
Significantly increased frequencies of micronuclei were shown in active
phase RA disease (both early and long-term) but not in the group with low
disease activity, as compared to controls. Chromosome aberrations were
detected for all 4 RA groups. The highest frequencies of micronuclei and
chromosome aberrations were found in the long-termactive RA group. After 3
weeks-treatment, there were no significant decrease of the micronuclei
frequencies compared to baseline, although they were reduced in all RA
groups, except for the group with the long-term active disease. High level
of cytogenetic damage in RA patients was concordant with duration and
activity of the RA disease. At 3 weeks of therapy, neither the combined
treatment (MTX+DOLE), nor MTX alone did not affect the frequency of
micronuclei formation. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. OI 173034]
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