Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study

Živković, Lada; Bajić, Vladan; Bruić, Marija; Borozan, Sunčica; Popic, Kristina; Topalović, Dijana; Santibanez, Juan F; Spremo-Potparević, Biljana

doi:10.1016/j.mrgentox.2019.06.008

Cited by 15 publications

(15 citation statements)

References 27 publications

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“…Based on previous research articles, bioactive compounds originating from mushroom species such as phenolics (phenolic acids and flavonoids), [2,3,6,7,30] polysaccharides and proteins [4,34] or polysaccharide-protein complexes [5,21] contribute significantly in their antioxidant potential of mushroom species. The obtained results for the contents of TP, TF and TPR are shown in Table 2.…”

Section: Determination Of Total Phenol Total Flavonoid and Total Promentioning

confidence: 99%

“…[28] Also, hydrogen peroxide (H 2 O 2 ) causes DNA damage through oxygen radical mechanisms that can generate multiple DNA modifications. [4] Compounds with antioxidant potential, may be either simple secondary metabolites (such as, phenolic acids and flavonoids) or highly polymerized compounds (e. g., lignins, melanins, tannins) and inhibit free radicals production or alternatively remove them. [28] Most often, extracts that contain more phenolic compounds express better antioxidant and/ or cytotoxic activities.…”

Section: Introductionmentioning

confidence: 99%

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Bioactive Phenolic Compounds of Two Medicinal Mushroom Species Trametes versicolor and Stereum subtomentosum as Antioxidant and Antiproliferative Agents

Rašeta

Popović

Knežević

et al. 2020

Chemistry & Biodiversity

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Medicinal mushrooms have tremendous potential in production of bioactive compounds with diverse bioactivities while the biochemical potential of some specific mushroom strains (autochthonous for the region) in production of specific bioactive agents may be of the main importance in a continuous search for novel strains with supreme activities all over the world. In this study, the ethanolic (EtOH) and water (H 2 O) extracts of wild-growing polypore mushroom species were investigated: Trametes versicolor (L.) Lloyd and Stereum subtomentosum Pouzar. This study was designed to determine total phenol (TP), flavonoid (TF) and protein content (TPR) as well as LC/MS/MS phenolic profile related to in vitro antioxidant, antiproliferative (MTT assay) (AP) and DNA fragmentation properties. The H 2 O extracts expressed better antioxidant scavenging potential than EtOH showing the highest activity for the T. versicolor (IC 50 = 5.6 μg/mL, IC 50 = 0.6 μg/mL for DPPH * and OH * , respectively) while O 2 *À activity achieved the best activity for S. subtomentosum (IC 50 = 4.1 μg/mL). In contrary, the highest AP activity was obtained for the EtOH extracts of S. subtomentosum (IC 50 = 141.1 μg/mL). The EtOH extracts of both species showed the highest TP, TF and TPR content. Obtained results of DNA degradation indicate genotoxicity potential of the extracts at high concentration. The LC/MS/MS detection showed that the majority of analyzed extracts contained phenolic acids, p-hydroxybenzoic and protocatechuic acid. The obtained results suggest that analyzed medicinal mushroom species, T. versicolor and S. subtomentosum, could be of potential interest as new sources of strong natural antioxidants as well as antiproliferative agents in the future.

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Section: Determination Of Total Phenol Total Flavonoid and Total Promentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioactive Phenolic Compounds of Two Medicinal Mushroom Species Trametes versicolor and Stereum subtomentosum as Antioxidant and Antiproliferative Agents

Rašeta

Popović

Knežević

et al. 2020

Chemistry & Biodiversity

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“…A significant antigenotoxic effect was determined on the interventional level in the cotreatment experiment. This could be explained by an additional activation of independent mechanisms for making the cell genome more resistant to oxidative damage in the presence of tested compounds: free radical scavenging, enhancement of the cell antioxidant capacity, and stimulation of cell's DNA repair [27].…”

Section: Discussionmentioning

confidence: 99%

Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H₂O₂-Induced DNA Damage in Human Whole Blood Cells

Živković

Bajić

Topalović

et al. 2019

Oxidative Medicine and Cellular Longevity

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The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0.05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.

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“…Rezultati ove studije tokom koje je izvršena evaluacija uklanjanja hidroksil radikala C. sinensisa (IC50 = 0,5 mg/mL), idu u prilog njegovoj snažnoj antioksidativnoj sposobnosti. S druge strane, treba pomenuti da su naša prethodna istraživanja pokazala da Agaricus blazei TM (37) i Immune assist TM (dijetetski proizvod sastavljen od šest vrsta različitih medicinskih gljiva među kojima je i C. sinensis) (38) imaju sličnu aktivnost uklanjanja hidroksil radikala (IC50 = 0,2 mg/mL). Takođe, redukciona sposobnost C. sinensisa umerena je u odnosu na BHT kao standard, ali u istom opsegu kao kod Immune assista TM , dok je Agaricus blazei TM imao dva puta veću redukcionu sposobnost.…”

Section: Diskusijaunclassified

“…Takođe, redukciona sposobnost C. sinensisa umerena je u odnosu na BHT kao standard, ali u istom opsegu kao kod Immune assista TM , dok je Agaricus blazei TM imao dva puta veću redukcionu sposobnost. DPPH aktivnost C. sinensisa je izostala, slično kao i kod Immune assista TM i Agaricus blazei TM (37,38). S obzirom na to da je kod C. sinensisa izostala antioksidativna sposobnost uklanjanja DPPH radikala, treba izvršiti dalju analizu primene ove analitičke metode zbog sternih smetnji i veličine samog radikala, što predstavlja jednu od negativnih strana primene pomenutog testa.…”

Section: Diskusijaunclassified

Evaluation of antioxidant potential of Cordyceps sinensis in vitro

Živković¹,

Borozan²,

Bajić³

et al. 2019

Medicinski časopis

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Pozitivni uticaj konzumiranja prirodnih proizvoda na zdravlje ljudi odavno je poznat. Rasprostranjeno je mišljenje da ishrana ima značajnu ulogu u održavanju dobrog zdravlja, ali da takođe može da doprinese razvoju mnogih hroničnih bolesti (1). Gljive se od davnina cene zbog specifičnog ukusa i arome, ali i zbog farmakoloških svojstava (2, 3). Cordyceps sinensis (C. sinensis) dobro je poznata kineska lekovita gljiva, čije je prirodno stanište na Tibetanskoj visoravni, na visinama iznad 3.600 m. C. sinensis je parazitska gljiva koja raste na larvama leptira reda lepidoptera, potpuno uništavajući domaćina, pretvarajući ga u plodonosno telo same gljive (4, 5). U tradicionalnoj kineskoj medicini, C. sinensis se koristi kao deo medicinskog tretmana za otklanjanje slabog apetita, tuberkuloze, hroničnog bronhitisa i muške impotencije (6). Takođe, dugi niz godina C. sinensis se primenjuje za pojačavanje vitalnosti i usporavanje procesa starenja, lečenje bolesti respiratornog trakta i bubrežne disfunkcije; lečenje zdravstvenih stanja koja se odnose na kardiovaskularni, imunski, endokrini i nervni sistem (7). ORIGINALNI NAUČNI ČLANAK SAŽETAK Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil)-skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.

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Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study

Cited by 15 publications

References 27 publications

Bioactive Phenolic Compounds of Two Medicinal Mushroom Species Trametes versicolor and Stereum subtomentosum as Antioxidant and Antiproliferative Agents

Bioactive Phenolic Compounds of Two Medicinal Mushroom Species Trametes versicolor and Stereum subtomentosum as Antioxidant and Antiproliferative Agents

Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H₂O₂-Induced DNA Damage in Human Whole Blood Cells

Evaluation of antioxidant potential of Cordyceps sinensis in vitro

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Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study

Cited by 15 publications

References 27 publications

Bioactive Phenolic Compounds of Two Medicinal Mushroom Species Trametes versicolor and Stereum subtomentosum as Antioxidant and Antiproliferative Agents

Bioactive Phenolic Compounds of Two Medicinal Mushroom Species Trametes versicolor and Stereum subtomentosum as Antioxidant and Antiproliferative Agents

Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells

Evaluation of antioxidant potential of Cordyceps sinensis in vitro

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Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H₂O₂-Induced DNA Damage in Human Whole Blood Cells