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This article focuses on simulation and comparison of line codes NRZ (Non Return to Zero), RZ (Return to Zero) and Miller’s code for NG-PON2 (Next-Generation Passive Optical Network Stage 2) using. Our article provides solutions with Q-factor, BER (Bit Error Rate), and bandwidth comparison. Line codes are the most important part of communication over the optical fibre. The main role of these codes is digital signal representation. NG-PON2 networks use optical fibres for communication that is the reason why OptSim v5.2 is used for simulation.