This study aimed to evaluate the performance of 46 maize lines (S4) obtained from crosses between the commercial hybrids Penta x P30F53 in topcrosses with the commercial simple cross hybrid Dow8460 (tester) and checks (hybrids Penta, P30F53, Dow8460 and Status). The grain yield was evaluated in two environments in Guarapuava, Paraná State, and the effects of genotype, environment and genotype x environment interaction were significant. The grain yield of the topcross hybrids ranged from 8,416 to 13,428 kg ha-1. The agronomic characteristics of the forage and the bromatological characteristics of the silage were evaluated in environment 1. The green mass yield of the forage ranged from 48,767 to 87,714 kg ha-1 and the dry mass yield ranged from 14,749 to 26,130 kg ha-1. The neutral detergent fiber content ranged from 44.85 to 58.45% and the acid detergent fiber content ranged from 28.28 to 37.06%. The relative feed value of the silage ranged between 100.5 and 138.5. The tester, hybrid Dow8460, was efficient to discriminate the relative performance of the S4 lines in the topcrosses.

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5, 10 and 20% (w:v) and incorporated into the PDA (potato dextrose agar) culture medium before sterilization by autoclaving. Later, an 8 mm diameter disc of each pathogen mycelium was transferred to the center of the Petri dishes. After 24, 48 and 96 hours of incubation in a growth chamber at 22 ± 2 °C and a photoperiod of 12 hours, we evaluated the mycelial growth of F. moniliforme, and C. gloesporioides. In the last period of incubation, we quantified the production of conidia of each fungus. For the germination test, we added, into the wells of an ELISA test plates, a 40 μL aliquot of each extract at the concentrations of 0, 5, 10 and 20% and another aliquot of a suspension of conidia of each pathogen. After 24 hours at 22 ± 2 °C in the dark, the germination of the fungi was stopped with the addition of 20 μL of lactophenol. Then, we evaluated the germination of conidia. The experiments

ResumoO objetivo do trabalho foi avaliar o comportamento relativo de 74 linhagens parcialmente endogâmicas (S 4 ) provenientes do cruzamento entre os híbridos comerciais P30P70 e Dow8460 em topcrosses com o híbrido simples comercial P30F53 (testador), mais as testemunhas (híbridos Dow8460, P30F53 e Status). A produtividade de grãos (PG) foi avaliada em dois ambientes em Guarapuava-PR. Houve efeito significativo de genótipos, ambientes e da interação genótipos x ambientes quanto à produtividade de grãos (PG AbstractThe objective of this study was to evaluate the relative performance of 74 partially inbred lines (S 4 ) from crosses between the commercial hybrids P30P70 and Dow8460 in topcrosses with the commercial single cross hybrid P30F53 (tester), and checks (hybrids Dow8460, P30F53 and Status). The grain yield (GY) was evaluated in two environments in Guarapuava-PR. The effects of genotypes, environments

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