Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine.
Recurrent laryngeal neuropathy (RLN) etiology can be acquired, iatrogenic or idiopathic. There are no previous reports of RLN caused by recurrent laryngeal nerve compression by melanomas. This report describes a horse presenting severe dyspnea and progressive weight loss. Physical exam demonstrated tachycardia, tachypnea, inspiratory dyspnea at rest, neck extension and mydriasis. Temporary tracheotomy was performed and videoendoscopic examination diagnosed grade IV laryngeal paralysis. The animal came suddenly to death by suppurative bacterial pneumonia. At necropsy, it was possible to observe multiple melanotic epithelioid melanoma nodules compressing the recurrent laryngeal nerve, alongside with lung and parotid metastasis. This finding emphasizes the importance of establishing a differential diagnosis for tumor mass compression in the etiology of RLN, especially melanomas in gray horses, with or without cutaneous manifestations of masses.
Resumo: O objetivo deste estudo foi realizar a avaliação clínica e ultrassonográfica de uma metodologia de indução de tendinite do tendão flexor digital superficial (TFDS) em equinos. Foram utilizados oito equinos adultos, hígidos submetidos a indução de uma lesão realizada com suspensão de colagenase em gel, que foi administrada na região central média do TFDS com o auxílio do aparelho de ultrassom. Os animais foram acompanhados durante 15 dias pós-indução, onde foram realizados exames clínicos e ultrassonográficos. Foi evidenciada claudicação em todos os animais. Na avaliação ultrassonográfica pode ser confirmada a tendinite do TFDS. A técnica adotada para a indução experimental da tendinite em equinos demonstrou ser efetiva e poderá ser utilizada para aperfeiçoar a execução de novos estudos envolvendo o tratamento de tendinites experimentais induzidas.Palavras-chave: colagenase, gel, lesão, tendão. New model of equine tendinitis induction -clinical and ultrasound evaluationSummary: The objective of this study was to evaluate clinically and ultrasonography a tendinitis new induction methodology of superficial digital flexor tendon (SDFT) in horses. Eight adult horses clinically healthy were used. The injury induction was performed with a collagenase suspension in gel, which was administered at the central SDFT medium with the use of ultrasound. The animals were followed for 15 days post-induction, where physical and ultrasound examinations were performed. Lameness was also evidenced in all animals and SFDT lesion could be confirmed by ultrasound analysis. The technique adopted for the experimental induction of tendinitis in horses proved to be effective and may be used to improve the implementation of new studies involving the treatment of experimentally induced tendinitis.
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