Background Maternal periodontal disease leads to low birth weight (LBW), insulin resistance (IR), increased TNF‐α levels, and alterations in insulin signaling in adult offspring. TNF‐α has been associated with the stimulation of IKKβ/NF‐κB, resulting in the decreased expression of GLUT4. Another mechanism that may be involved in decreasing GLUT4 expression is DNA methylation. This study aimed to evaluate in the adult offspring of rats with periodontal disease: IR, inflammatory pathways, DNA methylation, and expression of GLUT4. Methods Female Wistar rats were distributed into control and experimental periodontal disease groups. Seven days after induction of periodontal disease, both groups were mated with healthy male rats. After weaning, male offspring were distributed into control offspring (CN‐o) and periodontal disease offspring (PED‐o) groups. Body weights were measured from 0–75 days of age. At day 75, the following were measured in the offspring: IR (HOMA‐IR index); TNF‐α and NF‐κBp65 content in the gastrocnemius muscle (GM) by western blotting; IKKα/β, JNK, ERK 1/2, NF‐κBp65, and NF‐κBp50 phosphorylation status in the GM by western blotting; DNA methylation by restriction digest and real‐time PCR(qAMP); and expression of GLUT4 mRNA in the GM by real‐time PCR. Results LBW, IR, increases in TNF‐α, IKKα/β, ERK 1/2, NF‐κBp65, and NF‐κBp50 decreased expression of GLUT4 mRNA were observed in the PED‐o rats. No differences were identified in JNK phosphorylation status and DNA methylation in the evaluated regions of the GLUT4‐encoding gene Slc2a4. Conclusion Maternal periodontal disease causes LBW, IR, activation of inflammatory pathways, and decreased GLUT4 expression in the GM of adult offspring.

The rating of perceived exertion (RPE) is ability to detect and interpret organic sensations while performing exercises. This method has been used to measure the level of effort that is felt during weight-training at a given intensity. The purpose of this investigation was to compare session RPE values with those of traditional RPE measurements for different weight-training muscle actions, performed together or separately. Fourteen women with no former weight-training experience were recruited for the investigation. All participants completed five sessions of exercise: familiarization, maximum force, concentric-only (CONC-only), eccentric-only (ECC-only), and dynamic (DYN = CONC + ECC). The traditional RPE method was measured after each series of exercises, and the session RPE was measured 30 min after the end of the training session. The statistical analyses used were the paired t-test, one-way analysis of variance, and repeated measures analysis of variance. Significant differences between traditional RPE and session RPE for DYN, CONC, and ECC exercises were not found. This investigation demonstrated that session RPE is similar to traditional RPE in terms of weight-training involving concentric, eccentric, or dynamic muscle exercises, and that it can be used to prescribe and monitor weight-training sessions in older subjects.

RESUMOIntrodução: Sensações de prazer/desprazer são moduladas de acordo com as intensidades prescritas para o exercício, demonstrando uma relação simples, onde quanto mais intenso o estímulo, maior o esforço percebido. Objetivo: Verificar as diferenças entre as respostas perceptuais e afetivas agudas em diferentes intensidades de exercícios com pesos em mulheres idosas. Métodos: Quatorze mulheres com idade entre 65 e 75 anos, previamente sedentárias, com sobrepeso, foram submetidas a três diferentes intensidades de treinamento com pesos: 35% e 70% de 1RM, e autosselecionada. Foram avaliados o afeto e a percepção subjetiva do esforço (PSE). Após 30 minutos do término da sessão, foi avaliada a percepção subjetiva do esforço da sessão (PSE-S). Foi utilizado o teste de variância One-way ANOVA de um fator de medidas repetidas para as variáveis dependentes PSE-S, PSE e afeto, seguido de post hock de Tukey. Resultados: A PSE-S demonstrou diferença significativa da testagem (F (7,99) =15,358; p=0,001) entre as intensidades, assim como para PSE entre cada exercício. A PSE-S, para 35% de 1RM, foi menor, quando comparada à PSE-S para 70% de 1RM, e à intensidade autosselecionada. A intensidade de 70% de 1RM, quando comparada com a intensidade autosselecionada, não apresentou diferença significativa. Em relação a PSE nos exercícios, foi observado um esforço significativamente maior (p < 0,001) para 70% de 1RM, quando comparados com a autosseleção da carga. O afeto revelou diferença significativa na intensidade de 35% de 1RM, mostrando uma resposta mais prazerosa, quando comparado à carga de 70% de 1RM, e à autosseleção da carga. Conclusão: Os resultados demonstraram que baixas intensidades são percebidas como menor esforço e este fato contribuiu para a produção de respostas afetivas mais prazerosas. Além disso, fatores interindividuais são capazes de modular estas respostas, possibilitando a diminuição da percepção do esforço em intensidades mais vigorosas. (F (7.99) = 15.358; p = 0.001) Palavras

[Purpose] The aim of the present study was to verify the rate of perceived exertion and feelings of pleasure/displeasure in elderly women, who did normally perform physical exercises, following eight weeks of strength training in a constant routine. [Subjects and Methods] Eleven sedentary women were subjected to anthropometric assessment. The maximum load (100%) for each used in this study was determined by performing a test to determined the 1RM for each of them according to the protocol of Fatouros et al. and the Feeling Scale and RPE scale were explained to the women. After these initial procedures, the subjects followed a routine for strength training, performing three sets of repetitions at 70% of the one-repetition maximum for each exercise (bench press, leg extension, pulldown, leg curl) without modifying the exercises and their execution order. The frequency of training was three days per week. ANOVA was used to analyze the behavior of the dependent variable, and the post hoc tests were used to identify significant differences. [Results] Strength increased only in the fifth week. The rate of perceived exertion showed a reduction only in the fifth week in the leg extension, pulldown, leg curl. [Conclusion] The percentage of 70% the one-repetition maximum recommended to increase the strength gains and hypertrophy of skeletal muscle does not provide feelings of displeasure when performing proposed exercise. However, it may be possible to modulate this percentage to obtain more pleasant feelings over two months.

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