Background: Arsenic is highly toxic agent and a risk factor for disease and disability. Arsenic is present in drinking water of many developing and developed countries including Pakistan and due to rapid industrialization its quantity in soil and water is increasing day by day.Methods: In an 18 month study in which we took two principal groups, labelled as control group A and experimental group B. The animals of experimental group B were administered 4 µg of sodium arsenite dissolved in 10 ml of distilled water by oral gavage daily for 14 days. The uterus was removed and processed for paraffin embedding and stained with hematoxylin and eosin (H and E). The histological parameters; uterine luminal diameter, height of uterine luminal epithelium, area occupied by epithelial component of uterine glands and the thickness of myometrium were measured and evaluated by civil AutoCAD 2013 software. The data was analyzed statistically with the statistical package for social sciences (SPSS).Results: Histological results showed the degenerative effects. The luminal diameter of uterine horns was reduced in experimental animals. The height of uterine epithelium was reduced. Area occupied by epithelial component of uterine glands was reduced along the reduction in the thickness of myometrium.Conclusions: The histological abnormalities observed in uterus showed that the degenerative effects may be due to oxidative stress produced by the exposure to sodium arsenite. As sodium arsenite produces the oxidative stress by the formation of free radicals and by the denaturation of proteins.
Arsenic contamination among heavy metals in ground water is a health hazard due to its toxicological concern, affecting millions of humans globally. Purpose: To observe and analyze the histomorphological effects of sodium arsenite on cervix of rat. Study Design: Laboratory based experimental study. Methodology: Present study held at department of anatomy, College of Physician and Surgeon Pakistan, Regional Centre Islamabad, in two principal groups, group A (control) and group B (experimental). The animals of B were administered sodium arsenite by oral gavage daily for 14 days while group A were administered only distilled water daily for 14 days. The animals of both groups were sacrificed after day 14th of drug administration. The cervix was processed for paraffin embedding and stained with Haematoxylin and Eosin (H&E). Statistical analysis: Data analyzed by SPSS 22.0v. Student’s t-test was applied to determine the significance. P-value ≤ 0.05 was considered significant. Results: The histological evaluation of experimental group animals showed decrease in cervical canal’s luminal area, hyperplasia of cervical epithelium and decrease thickness of cervical muscles. Conclusion: The nature of histomorphological effects observed in cervix showed that these changes may be due to oxidative stress produced by the formation of free radicals, decrease levels of serum estradiol, progesterone and by the denaturation of proteins. Key Words: Arsenic, Cervix, Free Radicals, Hyperplasia and Oxidative stress.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.