Plants are often subjected to periods of soil and atmospheric water deficit during their life cycle. The frequency of such phenomena is likely to increase in the future even outside today's arid/semi-arid regions. Plant responses to water scarcity are complex, involving deleterious and/or adaptive changes, and under field conditions these responses can be synergistically or antagonistically modified by the superimposition of other stresses. This complexity is illustrated using examples of woody and herbaceous species mostly from Mediterranean-type ecosystems, with strategies ranging from drought-avoidance, as in winter/spring annuals or in deep-rooted perennials, to the stress resistance of sclerophylls. Differences among species that can be traced to different capacities for water acquisition, rather than to differences in metabolism at a given water status, are described. Changes in the root : shoot ratio or the temporary accumulation of reserves in the stem are accompanied by alterations in nitrogen and carbon metabolism, the fine regulation of which is still largely unknown. At the leaf level, the dissipation of excitation energy through processes other than photosynthetic C-metabolism is an important defence mechanism under conditions of water stress and is accompanied by down-regulation of photochemistry and, in the longer term, of carbon metabolism.
Potted cuttings of three Eucalyptus globulus Labill. clones (AR3, CN44, MP11) were either well watered or subjected to one of two soil water deficit regimes for six months in a greenhouse. Reductions in lateral branching, leaf production and leaf expansion were the leading contributors to the large differences observed in biomass production between well-watered and water-stressed plants. Although no significant differences among clones were observed in dry matter accumulation or in the magnitude of the response to soil water deficits, sensitivity of lateral branching, leaf initiation and whole-plant foliage to water stress was significantly lower in CN44 than in AR3 and MP11. When the confounding effect of differences in plant size resulting from the different watering regimes was removed, allometric analysis indicated that the genotypes differed in biomass allocation patterns. In addition to a drought-induced reduction in leaf number, water deficits also resulted in smaller leaves because leaf expansion was inhibited during dehydration events. Resumption of leaf expansion following stress relief occurred in all of the clones, but was particularly evident in severely stressed plants of Clone AR3, possibly as a result of the osmotic adjustment observed in this genotype.
Potted cuttings of three Eucalyptus globulus Labill.
clones (AR3, CN44, MP11) were grown over 6 months in a greenhouse under three
watering regimes: well watered (HW), moderate soil water deficit (MS) and
severe soil water deficit (SS). Transpiration efficiency
(W = total dry matter/water transpired) and
leaf intrinsic gas exchange efficiency
(A/gs = carbon assimilation
rate/stomatal conductance) increased under water stress and were
positively correlated with the stable carbon isotope composition of leaf
tissue (δ13C). The clones did not vary
significantly with respect to A/gs
and W. However, statistically significant differences were detected among
clones in δ13C, A and
biomass. W did not differ between the MS and SS regimes,
probably due to plant acclimation to increasing soil water deficits. The
increase in W with soil water deficits relative to the
well watered control was primarily associated with stomatal closure, but was
also influenced by differences in respiratory carbon losses
(?c) and variation in the leaf-to-air water vapour
difference (v). Variance in
?c and v may explain
partially why the two levels of soil water deficit were different in regard to
δ13C but not in terms of W.
The authors aknowledge Environmental and Experimental Botany, an Elsevier Journal.
AbstractThe effects of a slow-imposing two-weeks soil drying period, and subsequent re-watering, on leaf water potential (Ψ), gas exchange rates, chlorophyll fluorescence and on the concentrations of malondialdehyde (MDA) and non-structural carbohydrates
The effects of a two weeks soil drying period on the activity of nitrate reductase (NR; EC 1.6.6.6) were studied on Helianthus annuus L. and non-nodulated Lupinus albus L. plants, growing under two nutrient supply regimes. NR activity was assessed in leaf and root extracts, by measuring the activity of the unphosphorylated active form (NR act ), the maximal extractable activity (NR max ) and the activation state. To get insight into potential signalling compounds, nitrate, amino acids and soluble sugars concentrations were also quantified. On both species, foliar NR act and NR max were negatively affected by soil drying and reduced supply of nutrients, the observed changes in NR activity being linearly-correlated with the depletion of nitrate. Similar results were obtained in the roots of sunflower. Conversely, in white lupin roots NR max was found to be independent of tissue nitrate concentration. Regardless of the species and organ, the activation state of the enzyme was unaffected by the nutrient supply regime. In well-watered sunflower roots only about 50% of the existing NR was unphosphorylated, but the activation state increased significantly in response to drought. In contrast, lupin roots always exhibited NR activation state values close to 80% or even higher. At the leaf level, NR activation state was hardly changed in response to soil drying. The contribution of changes in the concentrations of soluble 5 sugars and amino acids to explain the observed variations in NR activity are discussed.
AbbreviationsFW, Fresh weight; NR, Nitrate reductase; NR act , activity of the unphosphorylated form of nitrate reductase; NR max , maximal extractable activity of nitrate reductase
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