We analyzed changes in land cover in the Sierra de San Javier and its surroundings, an area of ca. 70 000 ha near San Miguel de Tucumán, an urban center of ca. 1 million people in subtropical Argentina. The analysis covered two periods: 1949-1972 and 1972-2006 using remote sensing techniques. For the year 2001, we mapped the patterns of distribution of secondary forests dominated by the most abundant exotic tree species (Ligustrum lucidum). Based on land-cover maps, we estimated sediment yield as an index of watershed condition. Urban area was growing during the whole study period. Between 1949 and 2006, forest area increased approximately 1400 ha, mostly over abandoned agriculture and grasslands; this expansion was accelerated between 1972 and 2006. Increased forest cover resulted in a reduction in erosion and sediment yield that was disproportionately large, as most new forests are located in areas of steep slopes and high rainfall. By 2001, Ligustum-dominated forests had expanded to more than 500 ha, in the southern portion of the sierra only. Overall, the analysis quantifies a process of Neotropical periurban forest transition, likely associated with socioeconomic changes related to population urbanization, that promotes improvements of some environmental services, such as watershed and biodiversity conservation. However, natural communities are strongly affected by past land use and neighboring urban areas, which have promoted a growing importance of exotic species with mostly unknown ecological consequences.

f Chronic biofilm infections are often accompanied by a chronic inflammatory response, leading to impaired healing and increased, irreversible damage to host tissues. Biofilm formation is a major virulence factor for Candida albicans and a challenge for treatment. Most current antifungals have proved ineffective in eradicating infections attributed to biofilms. The biofilm structure protects Candida species against antifungals and provides a way for them to evade host immune systems. This leads to a very distinct inflammatory response compared to that seen in planktonic infections. Previously, we showed the superior efficacy of DL-2-hydroxyisocaproic acid (HICA) against various bacteria and fungi. However, the immunomodulatory properties of HICA have not been studied. Our aim was to investigate the potential anti-inflammatory response to HICA in vivo. We hypothesized that HICA reduces the levels of immune mediators and attenuates the inflammatory response. In a murine model, a robust biofilm was formed for 5 days in a diffusion chamber implanted underneath mouse skin. The biofilm was treated for 12 h with HICA, while caspofungin and phosphate-buffered saline (PBS) were used as controls. The pathophysiology and immunoexpression in the tissues surrounding the chamber were determined by immunohistochemistry. Histopathological examination showed an attenuated inflammatory response together with reduced expression of matrix metalloproteinase 9 (MMP-9) and myeloperoxidase (MPO) compared to those of chambers containing caspofungin and PBS. Interestingly, the expression of developmental endothelial locus 1 (Del-1), an antagonist of neutrophil extravasation, increased after treatment with HICA. Considering its anti-inflammatory and antimicrobial activity, HICA may have enormous therapeutic potential in the treatment of chronic biofilm infections and inflammation, such as those seen with chronic wounds.

A water and sanitation sustainability index (WASSI) was developed and estimated in four cities of the province of Salta, in northern Argentina. The index was built with nine descriptors and fifteen indicators that covered all essential aspects of the sustainability of local water and sanitation management systems. Only one of the cities studied obtained a sustainability value above the acceptability threshold adopted (50 of 100 points). Results indicate that the water company needs to address some environmental and social issues to enhance the sustainability of the systems studied. The WASSI was conceptually robust and operationally simple, and could be easily adapted to the case studies. The index can be followed and updated online on a web site specially developed for this project. This website could be useful to promote participatory processes, assist decision makers, and facilitate academic research. According to local stakeholders, a more open sustainability assessment based on sustainability indices and supported by virtual tools would be relevant and highly feasible. It would help decision makers improve the sustainability and transparency of water and sanitation management systems, and promote more sustainable water policies in the region and beyond. OPEN ACCESSSustainability 2015, 7 14538

The middle initials of the sixth and tenth authors were omitted. The correct names are Katherine A. Radek and Richard L. Gallo.