Aim. To evaluate the efficacy of Brazilian green propolis in comparison to miconazole gel in the treatment of Candida-associated denture stomatitis. Methods. Forty-five denture stomatitis patients, with palatal mucosa erythema levels classified according to Newtons's criteria and with positive culture to Candida spp., were randomly divided into three treatment groups: 15 received miconazole gel 2%, 15 received propolis gel 2,5%, and 15 received propolis 24% for mouthwash. After four daily use lasting two weeks, they were reexamined for the denture stomatitis degree and for a second culture of Candida. The Wilcoxon's test was applied to compare the results of clinical classification of the denture stomatitis and the Candida spp. colonies numbers, before and after each treatment. The Kruskall-Wallis's test was used to compare efficacy among the three treatment groups. Results. There were a significant reduction or complete remission of denture stomatitis (P < 0.05) and a significant decrease of Candida colonies for the three groups (P < 0.05). There was no difference in the efficacy among the treatment groups (P > 0.05). Conclusion. Brazilian green propolis has a similar effect as miconazole in the treatment of Candida-associated denture stomatitis being an alternative in the therapeutics of this condition.

No abstract

No abstract

RESUMOIntrodução: A técnica incremental restauradora, com aplicação de porções de resina composta nos preparos cavitários odontológicos, pode gerar o risco de infecção cruzada, uma vez que a resina é retirada de um mesmo tubete, que é utilizado em vários pacientes diferentes. Esse problema se agrava em clínicas acadêmicas de Odontologia, onde esses artigos são compartilhados por todos os alunos. De forma similar, o pincel de pelo Marta e a seringa para aplicação de ácido também podem apresentar microrganismos adquiridos durante o atendimento restaurador. Método: Diante desses eventuais riscos, este trabalho teve como propósito verificar a contaminação microbiológica de tubetes de resina, seringas de ácido e pincéis de pelo Marta através de amostras coletadas nas clínicas de Odontologia de uma universidade privada. Foram utilizados 23 tubetes de resina, 13 pincéis de pelo Marta e 13 seringas de ácido, divididos entre grupos caso e controle. Realizou-se o processamento através da fricção com "swab" estéril, nas partes externas das seringas e dos tubetes e da imersão dos pelos dos pincéis em solução salina por um minuto. Os espécimes foram semeados em caldo BHI e, após o crescimento, foram executadas diluições seriadas e semeaduras em meio Ágar Mitis salivarius, BHI e Hipertônico Manitol. Resultados e conclusões: Através da avaliação microbiológica qualitativa (avaliação da presença de turbidez) e quantitativa (número de UFC) de microrganismos, observou-se a contami nação de 50% dos pincéis e 46,15% dos tubetes, nos grupos caso; enquanto nas seringas com ácido e em todos os artigos do grupo controle não houve crescimento de microrganismos. DESCRITORES: Contaminação, Resinas compostas, Tubetes de resina, Pincel pelo Marta, Dentística. ABSTRACT Introduction:The layering composite technique which consists of applying small amounts of composite resin in the tooth cavity preparation, can generate the risk of cross infection. The contamination is due to removal of the composite of a single tube which, in turn, is used in many different patients. This problem increases in graduate dental clinics where many students practice together the technique of teeth restoration, using composite tubes that are shared by all them. The use of fur brushes and syringes for applying acid in different patients are also tools that may present microorganisms acquired during restorative procedures. Method: Considering this eventual risk, this study intended to verify the microbiological contamination of tubes of composite resin, syringe of acid and fur brushes in samples collected from dental clinics in a private university. The items used in this experiment were 23 tubes of resin, 13 fur brushes and 13 syringes of acid, and they were divided into case groups and control groups. Samples were collected with saline wetted swabs rubbed over the surfaces of each syringe and composite tube. The fur brushes were immersed in saline for 1 min. Later, the swabs were immersed in BHI broth and serially diluted samples were spread on Mitis salivarius, BH...

Aims:  This study was undertaken to detect, identify and determine antifungal susceptibility of yeast strains isolated from dental solid waste and to evaluate airborne fungi in the Brazilian dental health care environment and in the waste storage room. Methods and Results:  A group of 17 yeast strains were identified by macroscopic and microscopic characteristics, API 20C Aux system and Multiplex PCR. All 104 airborne fungal colonies were identified by macroscopic and microscopic morphology. The CLSI broth microdilution method was utilized as the susceptibility test. Candida parapsilosis was the prevailing yeast species recovered from waste, followed by Rhodotorula glutinis. Three strains of Candida guilliermondii presented minimal inhibitory concentration values considered to be susceptible dose dependent (2 μg ml−1) to voriconazole. Of all airborne fungal species, 69% were recovered from the waste storage room and 31% were recovered from the clinical/surgical environment. Most of them were identified as Cladosporium spp. Conclusions:  These findings reinforce the potential risk of waste handling and point out the need for safe management to minimize the spread of these agents to the environment. Filamentous fungi isolation in almost all sampled environments indicates that a periodic monitoring of airborne microbiota in the dental health care service environment is required. Significance and Impact of the Study:  The survival of yeast strains for 48 h suggests that dental waste should be carefully controlled and monitored.