The extensive geographic spread of MRSA isolates belonging to the Brazilian epidemic clone (BEC) limited the value of pulsed-®eld gel electrophoresis (PFGE) in epidemiological studies of outbreaks caused by these strains. Thus, the discriminatory power of eight different molecular methods was evaluated in an attempt to establish a methodology for genotyping BEC isolates involved in intra-hospital outbreaks. BEC isolates from ®ve hospitals in Teresina City, Piauõ Â State were genotyped by conventional electrophoresis or PFGE of Cla I-or Sma I-digested genomic DNA hybridised with speci®c labelled mecA, Tn554, IS257 and IS256 probes. The combination of PFGE with Cla I/mecA, Cla I/Tn554, Cla I/IS257, Sma I/mecA and Sma I/IS257 probe-®ngerprinting techniques provided a very poor discriminatory power for BEC strains. Although Cla I/ IS256 ®ngerprinting discriminated 17 different polymorphisms among the isolates displaying PFGE A 1 pattern, this strategy was not reproducible. In contrast, the combination of PFGE and Sma I/IS256 polymorphisms differentiated BEC isolates into nine stable polymorphisms. Thus combination of PFGE and hybridisation with IS256 probe may be recommended as a useful means of typing BEC strains involved in intrahospital infections.
Objective: To evaluate the distribution and susceptibility to antimicrobials of Shigella isolated from children with acute diarrhea and without diarrhea in Teresina, state of Piauí, Brazil. Methods:Four hundred children aged up to 60 months were studied. Stools were collected from all the patients between January 2004 and August 2007. Shigella was identified by conventional methods and antibiogram and extended-spectrum β-lactamase (ESBL) were performed by agar diffusion.Results: Shigellosis was only detected in children with acute diarrhea (26/250; 10.4%), especially in those aged from 6 to 24 months and in the rainy months. Shigella was susceptible to ceftriaxone, ciprofloxacin and nalidixic acid. More than half of the strains were resistant to sulphametoxazole-trimethoprim and ampicillin. ESBL was not detected. Conclusions:S. flexneri is common in Teresina. The resistance to ampicillin and sulphametoxazole-trimethoprim gives cause for concern, as these drugs are widely used in practice and sulphametoxazoletrimethoprim is also recommended for treating children suspected of having shigellosis. Resultados: Shigelose foi detectada apenas em crianças com diarreia aguda (26/250; 10,4%), especialmente naquelas entre 6 e 24 meses de idade e nos meses chuvosos. Shigella foi suscetível a ceftriaxona, ciprofloxacina e ácido nalidíxico. Mais da metade das amostras foram resistentes a sulfametoxazol-trimetoprim e ampicilina. ESBL não foi detectada. J Pediatr (Rio J)Conclusões: S. flexneri é comum em Teresina. A resistência a ampicilina e sulfametoxazol-trimetoprim é preocupante, pois estas drogas são amplamente utilizadas na prática e sulfametoxazol-trimetoprim ainda é recomendada para tratamento de crianças com suspeita de shigelose. J Pediatr (Rio J) IntroduçãoA enterite infecciosa constitui causa importante de morbimortalidade, especialmente nas regiões menos desenvolvidas do planeta 1-3 . Entre outros, Shigella é um dos principais agentes da doença 2 . Estima-se que mais de 160 milhões de seres humanos sejam infectados pelo microrganismo anualmente e que aproximadamente 1,1 milhão evoluam para o óbito 2 .
Justificativa e Objetivos: analisar a prevalência da prova tuberculínica positiva em estudantes de enfermagem de uma instituição pública de ensino superior. Métodos: estudo de delineamento transversal analítico em que foi utilizado um questionário de caracterização socioeducacional e, após o preenchimento do mesmo, os participantes (n=63) foram submetidos à prova tuberculínica, usando-se a técnica de Mantoux. Resultados: 36% dos participantes apresentaram reação positiva e que há associação estatisticamente significativa entre o semestre cursado pelos estudantes, o estágio em hospitais e a positividade para a prova tuberculínica. Conclusão: comprovou-se a alta prevalência de infecção latente da tuberculose em estudantes de enfermagem e associação significativa entre a prova tuberculínica positiva e o período cursado pelos estudantes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.