(1) Background: The Commercial Kit SIRE Nitratase ® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTEC TM MGIT TM 960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTEC TM MGIT TM 960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.
Comparamos o teste de nitrato redutase com o método de proporções, considerado como padrão ouro, em 57 cepas de Mycobacterium tuberculosis isoladas de pacientes atendidos no Hospital das Clínicas da Universidade Federal de Minas Gerais, em Belo Horizonte (MG). A sensibilidade, a especificidade e a acurácia para rifampicina e isoniazida foram de 100% para todas, enquanto essas foram, respectivamente, de 88,9%, 66,7% e 96,5% para estreptomicina e de 98,0%, 100% e 98,2% para etambutol. A média de tempo para a obtenção dos resultados foi de dez dias. Na amostra estudada, o teste de nitrato redutase mostrou grande acurácia e excelente concordância com o padrão ouro.
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