out of 268 lesions suggestive of CL were detected during ante mortem examination. Caseous lymphadenitis-like lesions were observed in all flocks examined from different municipalities in the states of Paraíba, Pernambuco and Bahia. Cultures and histologic examination were performed in samples from 51 lesions. Corynebacterium pseudotuberculosis was isolated in 74.5% (43/51) samples, Staphylococcus aureus in 7.8% (4/51) samples, Escherichia coli in 2% (1/51), and in 5.9% (3/51) samples cultures were negative. Histologic findings in 11 lymph nodes showed all characteristic lesions of caseous lymphadenitis: central area of necrosis formed by concentric lamellae, with large bacterial colonies and mineralization, surrounded by an inflammatory exudate with epithelioid macrophages and few neutrophils; lymphocytes and plasma cells were observed in the next layer, and fibrous tissue was surrounding the whole lesion. Lesions in the other 32 lymph nodes showed most, but not all lesions characteristic of the disease. Giant cells were observed in 21 lymph nodes. Histologic lesions in lymph nodes from which S. aureus was isolated were similar as those of CL. These findings suggest that CL lesions are characteristic, but not pathognogmonic, because they can be confused with lesions caused by other pyogenic bacteria or tuberculosis; therefore bacteriologic examination is necessary for the diagnosis of the disease.
The resurgence of visceral leishmaniasis in Brazil increases the need for studies to elucidate the spatial and temporal dynamics of Lutzomyia longipalpis (Lutz and Neiva) (Diptera: Psychodidae), the vector of Leishmania infantum, the causative agent of visceral leishmaniasis in Brazil. Sand flies were captured in peridomestic habitats biweekly for 3 yr. Cross-correlation tests and spectral analysis were used to analyze the simultaneous and lag-time correlations between Lu. longipalpis population densities and abiotic factors of temperature, relative humidity, wind velocity, and rainfall. Distinct seasonal patterns were observed for males and females, with intervals of 6 mo between population peaks for males and 12 mo for females. Peak female population densities lagged 3 mo behind the maximum annual temperature. Female population density was negatively correlated with relative humidity. An increase in average wind velocity was followed by a decrease in the number of females for 2 wk. Understanding the relationship between the seasonal population dynamics of Lu. longipalpis and abiotic factors will contribute to the design of better control measures to decrease transmission of L. infantum and consequently the incidence of leishmaniasis.
This study aimed to describe the first report of an outbreak of primary photosensitization in dairy goats caused by Froelichia humboldtiana. The disease occurred from March to May 2014, in a mixed flock of 15 Saanen, Alpine, and crossbred lactating goats. The entire flock was affected; however, the Saanen and cross-bred goats developed more severe lesions, and their milk production decreased by as much as 75%. Lesions were progressive and consisted of skin hyperemia, edema, and necrosis, especially in depigmented areas. An experimental study with two goats resulted in photodermatitis, similar to that observed in the natural cases, 10-14 days after the beginning of plant ingestion. Serum activities of aspartate aminotransferase (AST) and -glutamyltransferase (GGT) and, direct, indirect, and total bilirubin serum concentrations were within the normal ranges. In conclusion, F. humboldtiana causes primary photosensitization in goats with a negative impact on milk production.
This study investigated the gastrointestinal parasitism by helminths and protozoa in sheep (Ovis aries) Santa Inês breed, municipality of Lajes, Rio Grande do Norte. Monthly, from April 2005 to August 2007, stool samples were collected from two tracer lambs in the first day of the experiment and performed a necropsy of these animals in 44th day. A total of 64 lambs were sampled, but only 62 lambs were slaughtered. The fecal samples were examined by sedimentation in water. The contents of the abomasum, small intestine and large intestine were examined for the recovery of helminths. The parasitological examination revealed eggs of the following groups of helminths: Strongyloidea, Strongyloides sp., Trichuris sp., and Moniezia sp. Also were found oocysts of Eimeria spp., cysts of Entamoeba ovis and Giardia duodenalis. The helminths identified from examining the contents were: Haemonchus contortus, Cooperia pectinata, Cooperia punctata, Trichostrongylus colubriformis, Moniezia expansa, Oesophagostomum sp. Skrjabinema ovis and Trichuris sp.
Learning Objects (LO) are digital resources developed to help teachers present pedagogical concepts to students. However, the way these resources are produced does not allow teachers to adapt them to the reality of their students. Thus, this paper proposes a development process of customizable learning objects using a strategy based on models. Based on this approach, teachers will make adjustments to LO and thus achieve more autonomy in the use of these resources.
Session 1: Theme 2-Oral presentations labour, off-farm nutrients and sunlight to increase production and reduce losses to stresses, while preserving the environment. Effective deployment of AEl needs to be addressed for different production systems and conditions of market and input access. Homologue sites selected on the basis of agro-ecology, production systems and farming objectives were evaluated for the incidence of common pests and diseases. Interviews with 48 households in two production systems and agro-ecological zones documented knowledge for applying AEl, including identification of diseases. The interviews also inventoried available capital, labour, and land, including irrigation, manure and mulch. Elements of AEl were in use across sites. Subsistence farmers were constrained by lack of knowledge and resources, including labour, to effectively apply AEl practices, particularly households that sell their labour off farm. ln contrast, semicommercial farmers were motivated to seek and access knowledge and used their agricultural incomes to marshal resources such as labour to apply AEI. With regard to disease/pest symptoms, some farmers readily recognized certain diseases (e.g. Xanthomonas wilt), while others confused symptoms (Xanthomonas and Fusarium wilt). The study concludes that improved farmer knowledge and capacity for ecological reasoning willlead to more effective use of current on-farm resources for disease/pest and nutrient-cycle management. Households that sell their labour may have difficulty using labour-intensive AEI. Understanding ecology should allow more targeted use of labour for lPM and nutrient management.
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