The human T cell lymphotropic virus (HTLV) has a worldwide distribution. HTLV is endemic in some states in the northeastern region of Brazil. This study investigated the prevalence of HTLV-1/2 in 713 pregnant women attended at the Central Laboratory of Public Health of Maranhão (LACEN-MA) between February 2015 and May 2017. Serological screening was performed by chemiluminescent microparticle immunoassay (CMIA), and reactive sera were subsequently confirmed by Western blot (WB) analysis. Five samples were determined to be HTLV-1/2-reactive by CMIA analysis, while in the WB analysis, three sera were positive for HTLV-1, and two were indeterminate. The polymerase chain reaction (PCR) analysis used to detect HTLV-1 proviral DNA showed a specific 336 base pair fragment for HTLV-1 in all CMIA-reactive serum samples. PCR products were purified and sequenced. We observed a 0.7% molecular prevalence of HTLV-1 infection. The average age of the HTLV-1-positive pregnant women was 25.6 ± 8.2 years, and the average age of the HTLV-1-negative pregnant women was 24.3 ± 6.2 (p = 0.60). We observed that there was no association of HTLV-1 infection with age, ethnicity, marital status, educational level, family income, age of first sexual intercourse, previous pregnancy, breastfeeding, intravenous drug use by partner, history of blood transfusions, or use of condoms. The prevalence of HTLV-1 observed in pregnant women demonstrated the need to implement public health policies for the screening of HTLV-1/2 in prenatal care and counseling to avoid breastfeeding by infected women; this approach could control vertical transmission and reduce the spread of this virus in the population.

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Background: Brazil has a high prevalence of infections caused by different arboviruses. The standard method used for diagnosis is an Enzyme-linked immunosorbent assay for IgM capture (MAC-ELISA). This study aimed to optimize and evaluate a one-step reverse transcriptionpolymerase chain reaction to detect acute infections caused by dengue, zika, chikungunya, and mayaro virus in clinical samples. Methods: We evaluated 620 sera samples collected from March 2016 to March 2018 and provided by the Central Health Laboratory of Maranhão (LACEN-MA). Total RNA was isolated from clinical specimens and used as the template for one-step RT-PCR assays with specific-primers designed for this study. Results: Of the 620 sera evaluated, 386 (62.2%) were positive, among them 330 (85.5%) amplified a specific fragment for chikungunya, 55 (14.2%) showed a fragment compatible with dengue serotype 4, and 1 (0.3%) exhibited profile for mayaro virus. Conclusions: The results obtained here were more sensitive than IgM-ELISA because the viral RNA was detected in serum samples from patients, not only from 1 to 6 days but also from 7 to 10 days after the beginning of clinical signs (convalescent period). Besides, the mayaro virus was detected in one serum sample that was IgM-ELISA negative for dengue, zika, and chikungunya.

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Schistosomiasis is a serious parasitic infectious disease, one of the most important waterborne diseases, which is closely related to poverty, lack of health education and basic sanitation. It is a public health problem in Brazil, presenting endemic in some municipalities in the Maranhão State. In this way, this study aimed to evaluate the research profile of schistosomiasis mansoni in some endemic municipalities in the Maranhão State. A descriptive, retrospective and quantitative study was carried out with data obtained in “Programa de Controle da Esquistossomose (PCE) – MA” (Program for Schistosomiasis Control) between 2005 and 2015. We evaluated 42, 40 and 25 municipalities in the years 2005, 2010 and 2015, respectively. In 2005 the population evaluated was 184787, in 2010 162220 and in 2015 they were only 48484. The highest number of people evaluated in the year 2015 was in São Luís (8068) and the lowest was in Tutoia (83). The municipalities of Bacurituba, Guimarães, Guimaraes, Mirinzal, Paulino Neves and São João Batista presented a survey coverage of schistosomiasis major in the year 2015, compared to 2005 and 2010. Schistosomiasis remains a public health problem in the Maranhão State, however, its scope of research is still low, taking into account the number of endemic municipalities, sites susceptible to infection and the number of people examined.Key words: Schistosomiasis; Schistosoma mansoni; Maranhão.