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Arbuscular mycorrhizal fungi (AMF) comprise one of the main components of soil microbiota in most agroecosystems. These obligate mutualistic symbionts colonize the roots of most plants, including crop plants. Many papers have indicated that different crop management practices could affect AMF communities and their root colonization. However, there is little knowledge available on the influence of conventional and low-input agriculture on root colonization and AMF molecular diversity in rice fields. Two different agroecosystems (continuous conventional high-input rice monocropping and organic farming with a five-year crop rotation) and two different water management regimes have been considered in this study. Both morphological and molecular analyses were performed. The soil mycorrhizal potential, estimated using clover trap cultures, was high and similar in the two agroecosystems. The diversity of the AMF community in the soil, calculated by means of PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and 18S rDNA sequencing on clover trap cultures roots, was higher for the organic cultivation. The rice roots cultivated in the conventional agrosystem or under permanent flooding showed no AMF colonization, while the rice plants grown under the organic agriculture system showed typical mycorrhization patterns. Considered together, our data suggest that a high-input cropping system and conventional flooding depress AMF colonization in rice roots and that organic managements could help maintain a higher diversity of AMF communities in soil.

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Studies conducted at the field scale report significant reductions in the irrigation requirements of rice when continuous submergence (CS) is replaced by less water-demanding regimes such as flush-irrigation (FI, i.e. intermittent irrigations of rice growing in non-submerged soils). However, the effects of their extensive application in paddy areas with shallow groundwater is much less studied. We present a scenario analysis investigating the impacts on irrigation requirements induced by a shift from CS to FI in an irrigation district of Northern Italy where rice is the main crop, followed by maize and poplar. The area is characterised by a shallow water Table whose depth fluctuates between two meters (in winter) and less than 1 m (in summer). We applied a three-stage procedure, where we first analysed present state conditions using the SWAP (Soil, Water, Atmosphere, Plant) model to simulate irrigation deliveries and percolation fluxes. Then, we calibrated an empirical relationship between estimated percolation fluxes and measured depths to groundwater. Finally, we applied this relationship, in combination with the SWAP model, to predict the variation of district irrigation requirements due to a widespread shift from CS to FI. Results show that neglecting the feedback between groundwater recharge due to irrigation and groundwater depth led to overestimating the reduction of irrigation requirements of rice, which decreased from around 80% when no feedback was considered to around 60% when it was accounted for. Moreover, increased groundwater depths resulted in higher irrigation requirements for maize with an estimated growth of more than 50% due to the need of shortening the irrigation turn. These results demonstrate the importance of considering the impacts on the hydrological processes at larger scales when planning the conversion of CS into more efficient field irrigation methods.

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Temporal nitrogen (N) availability in fertilized rice paddies is the result of a balance of processes, mainly the gross rates of N mineralization, microbial and abiotic immobilization and N losses. Water and crop residue management practices often confound these established relationships making N the most difficult nutrient to manage in rice cropping systems. To investigate and quantify the interactive effects of soil redox conditions and straw incorporation on temporal fertilizer-N availability we treated a paddy soil with enriched ammonium-15 N and incubated for 160 days under flooded or non-flooded conditions, with or without the addition of rice straw. Changes in total available N as well as available and immobilized fertilizer-derived N (FDN) during incubation were evaluated. Under both oxic and waterlogged soils about 45-53% of applied N was rapidly immobilized. Whereas in the former most of this FDN was released contributing to the available N pool, flooded soils experienced significant losses from the soil/water system (≈ 67% of applied N). Addition of rice straw enhanced N immobilization, particularly under flooded conditions, that also contributed to limiting losses. Moreover, turnover of this labile organic matter pool supplied significant amounts of available N towards the later stages of the incubation, partly compensating for the immobilization of fertilizer-N.

Mycotoxigenic fungi and relative mycotoxins contamination were monitored in Italian paddy rice samples both in field during the growing season and the first five months of storage. Three experimental fields, nine rice varieties and three sowing densities were considered; then, different lots of paddy rice were stored in warehouses at different temperature regimes. Fusarium spp. and Aspergillus spp. were found to be the fungi most likely to produce mycotoxins throughout the growing season. In particular, A. flavus and A. niger were found only rarely both in field and in post-harvest, while A. versicolor was always present although in low concentrations. Penicillium spp. strains were isolated sporadically and were found to be irrelevant in Italian rice fungal contamination. Sterigmatocystin (STC) was the main mycotoxin found in Italian rice, while aflatoxin (AFB1), deoxynivalenol (DON) and ochratoxin A (OTA) were rarely detected. Contamination generally increased from post-flowering to ripening; considering rice varieties, significant differences (p ≤ 0.01) were found in fungal contamination and STC production; no differences were observed between sowing densities. During storage, an increase in STC content was observed in higher temperature regimes, while all the other considered mycotoxins remained unchanged. These results indicated that contamination by STC, an emerging mycotoxin not legislatively regulated by the European Union, can be relevant in rice.