We evaluated citric acid supplementation (0, 10, 20, and 30 g/kg) in plant protein‐based diets for juvenile tambaqui, Colossoma macropomum. In Experiment 1, fish (n = 160; 27.56 ± 2.73 g) were distributed in 310 L tanks (n = 16) for 80 days. Zootechnical performance, hematological parameters, and mineral composition (muscle and bones) did not differ significantly (p > .05). The inclusion of citric acid‐reduced pH of diets (p = .001) and stomach (p = .035). In Experiment 2, fish (n = 60; 48.00 ± 1.98 g) were distributed in fecal collection aquaria (200 L) for 21 days. The following were observed: a linear effect for the crude protein (p = .0004) and crude fat (p = .0037) digestibility coefficients; a quadratic effect for crude energy (p = .0037) and dry matter (p = .0004); an optimal supplementation level of 18.5 g/kg. Calcium (p = .0060) and magnesium (p = .0222) showed a linear effect, while phosphorus (p = .0001) had a quadratic effect with the 20 g/kg optimum supplementation level. To conclude, the supplementation of this organic acid in diets does not negatively affect fish nutritional status, health or welfare, and increases nutrient and mineral availability.
This study aimed to evaluate the yields of by-products and residues and the morphometry of juvenile tambaqui Colossoma macropomum undernutrition with different levels of lysine. Diets were elaborated containing 6. 60, 9.72, 12.84, 15.96, 19.08, and 22.20 g kg -1 of total lysine, and these were fed to fish distributed in 18 tanks (310 L each one) for 90 days. Morphometric analysis, measurements of whole fish, by-products, and residues were taken and compared to each other. All variables were validated using ANOVA, Tukey's test, and quadratic regression analysis (P < 0.05). No differences were found for the morphometric variables (P > 0.05). The ratio between morphometric data for the standard and total length showed significance at levels of 9.72 and 15.96 g kg -1 (P = 0.03). The gutted fish and fillets, the protein and lipid contents of the fillets, and the weights and lengths of the intestines did not change (P > 0.05). Moisture was modified with lysine at levels of 22.20 g kg -1 (P = 0.00), and minerals, between 6.60 and 22.20 g kg -1 (P = 0.01). The increase in lysine levels in the diet led to an increase in fin weight (P = 0.00). It was concluded that the addition of lysine in the diet did not directly influence the morphometry and yields of the fish in the juvenile phase, but it did affect the fin waste and the skin by-product.
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