Anaerobic digestion figures as a sustainable alternative to avoid discharge of cattle manure in the environment, which results in biogas and biofertilizer. Persistence of potentially pathogenic and drug-resistant bacteria during anaerobic digestion of cattle manure was evaluated. Selective cultures were performed for enterobacteria (ENT), non-fermenting Gram-negative rods (NFR) and Gram-positive cocci (GPC). Antimicrobial susceptibility patterns were determined and a decay of all bacterial groups was observed after 60days. Multidrug-resistant bacteria were detected both the influent and effluent. GPC, the most prevalent group was highly resistant against penicillin and levofloxacin, whereas resistance to ampicillin, ampicillin-sulbactam and chloramphenicol was frequently observed in the ENT and NFR groups. The data point out the need of discussions to better address management of biodigesters and the implementation of sanitary and microbiological safe treatments of animal manures to avoid consequences to human, animal and environmental health.

A minimal liquid medium containing benzene (B), toluene (T) and xylene (X) and mixtures thereof, was used to evaluate degradation activity of Pseudomonas putida CCMI 852 containing a TOL plasmid. Experiments were developed with B, T and X (100 mg L -1 ), with mixtures of BT, BX, and TX (50 + 50 mg L -1 each) and BTX (33.3 + 33.3 + 33.3 mg L -1 each), added to 500 mL of medium. After 18 to 24 hours, the inoculum was added and solvent disappearance was determined after 24 to 25 hours by GC. Results showed that P. putida CCMI 852 was able to metabolize T and X, but B was not metabolized. In a BTX mixture, B was not metabolized and T and X degradation rate decreased 50%.

Envi ronment noise pollution is common place today, at intolerable levels. In hospitals, technological developments have, as a consequence, potentially harmful noise levels. Much of the hospital noise comes from inside, rather than outside, and the major source of such noise is the Intensive Care Unit, for example equipment and hospital staff talk. Our goal with the present study was to investigate the noise level present in the different hospital environments, within a 222 bed hospital located at the 18th health zone, PR. Materials and Methods: The study was carried out in March, 2005, during a period of 24 hours, in tem different sectors. Case study: We checked environmental sound level by means of a model 1350 decibel meter. Results: The sound level found in our study was of 63.7 dB(A) in average, which exceeds the 45 dB recommended by the Brazilian Association of Technical Standards (1987). Conclusion: In the analyzed sectors, the sound level was considerably above the recommended maximum. The hospital staff should be aware of this noise level and its effects, so that they may act in a more efficient way in order to reduce this noise pollution; thus benefiting the professionals and patient recovery.

This is the first report to show AR gene dynamics and persistency of potentially pathogenic bacteria through metagenomic approach in cattle manure ambient temperature anaerobic digestion.

Scenedesmus obliquus (ACOI 204/07) microalgae were cultivated in cattle wastewater in vertical alveolar flat panel photobioreactors, operated in batch and continuous mode, after previous digestion in a hybrid anaerobic reactor. In batch operation, removal efficiencies ranges of 65 to 70% of COD, 98 to 99% of NH and 69 to 77.5% of PO after 12days were recorded. The corresponding figures for continuous flow were from 57 to 61% of COD, 94 to 96% of NH and 65 to 70% of PO with mean hidraulic retention time of 12days. Higher rates of CO fixation (327-547mgLd) and higher biomass volumetric productivity (213-358mgLd) were obtained in batch mode. This microalgae-mediated process can be considered promising for bioremediation and valorization of effluents produced by cattle breeding yielding a protein-rich microalgal biomass that could be eventually used as cattle feed.

Anaerobic digestion is an alternative method for the treatment of animal manure and wastewater. The anaerobic bioconversion of biomass requires a multi-step biological process, including microorganisms with distinct roles. The diversity and composition of microbial structure in pilot-scale anaerobic digestion operating at ambient temperature in Brazil were studied. Influence of the seasonal and temporal patterns on bacterial and archaeal communities were assessed by studying the variations in density, dynamic and diversity and structure. The average daily biogas produced in the summer and winter months was 18.7 and 16 L day(-1), respectively, and there was no difference in the average methane yield. Quantitative PCR analysis revealed that no differences in abundances and dynamics were found for bacterial communities and the total number of Archaea in different seasons. Analysis of bacterial clone libraries revealed a predominance of Firmicutes (54.5 %/summer and 46.7 %/winter) and Bacteroidetes (31.4 %/summer and 44.4 %/winter). Within the Archaea, the phylum Euryarchaeota was predominant in both digesters. Phylogenetic distribution showed changes in percentage between the phyla identified, but no alterations were recorded in the quality and amount of produced methane or community dynamics. The results may suggest that redundancy of microbial groups may have occurred, pointing to a more complex microbial community in the ecosystem related to this ambient temperature system.

RESUMO. Foram analisadas 413 amostras de água potável do sistema principal e do sistema secundário em Bandeirantes, Estado do Paraná, através de membrana filtrante para P. aeruginosa e bactérias heterotróficas em ágar Plate Count. Coliformes totais e fecais foram determinados pelo método dos tubos múltiplos. A qualidade físico-química da água foi determinada em termos de turbidez, pH, cloro, flúor, alcalinidade, cor, oxigênio dissolvido, dureza e matéria orgânica. Das amostras examinadas, 10,41% estavam contaminadas por P. aeruginosa, incluindo 23,53% das amostras do sistema de água secundário, e 8,56% das amostras do sistema de água principal. Somente uma amostra apresentou contagem de bactérias heterotróficas acima do nível máximo recomendado. P. aeruginosa isoladas foram testadas para sensibilidade ao cloro livre e sobreviveram a uma concentração de cloro três vezes acima da concentração mínima usada. É recomendada a determinação periódica de Pseudomonas em adição aos dados rotineiramente coletados na maioria dos sistemas de abastecimento.Palavras-chaves: Pseudomonas aeruginosa, análise de água, controle de qualidade microbiológica.ABSTRACT. Occurrence of Pseudomonas aeruginosa in drinking water. A total of 413 samples of drinking water, taken from principal and secondary water systems in the municipality of Bandeirantes, state of Paraná, southern Brazil, were analyzed through membrane filtration for P. aeruginosa and on Plate Count agar for heterotrophic bacteria. Total and fecal coliforms were determined by multiple dilution tube methods. Turbidity, pH, chlorine, fluoride, alkalinity, color, dissolved oxygen, hardness and organic matter were also measured. Of the 413 samples examined, 10.41% were contaminated by P. aeruginosa, including 23,53% of the water from secondary water system, 8,56% of the water from principal water system. No coliform was found in any of the samples. One sample counted above the maximum heterotrophic bacteria level permitted. P. aeruginosa isolated from the water samples were tested for chlorine sensitivity and survived up to three times the minimum chlorine concentration used. It is desirable that Pseudomonas enumeration be performed periodically, in addition to data routinely collected by most water supply systems.

This study aimed to analyze the economic viability of implementing and using a Canadian biodigester for power generation in a milk production system. Specifically, we intended to estimate the generated power production, the total production cost (TC), the total operating cost (TOC), and the effective operating cost (EOC) of 1 kW of power generated, and estimate the break-even point of the power produced. The research was carried out on a farm located in southern Minas Gerais (Brazil) from January to December 2017. Three scenarios were analyzed through the tree-point estimation (most likely, optimistic, and pessimistic). Scenario 1 considered the use of power for the acclimatization of free-stall barns during 13 hours day-1 plus the use of 50% of the produced biofertilizer. Scenario 2 considered the use of power during 13 hours day-1 for the acclimatization of free-stall barns and milking parlor, which means an increase in power consumption estimated at 10% compared to scenario 1, plus the use of 75% of the produced biofertilizer. Moreover, scenario 3 considered the use of power for the acclimatization of free-stall barns during 6.5 hours day-1 plus 25% of the produced biofertilizer. All scenarios considered the amount charged per kWh by the Companhia Energética de Minas Gerais (CEMIG). Monte Carlo simulations were carried out with minimum acceptable rates of return ranging from zero to 50%. Power generation was economically viable in all scenarios, with positive net present value (NPV), internal rates of return above the minimum acceptable rate of return, simple and discounted payback below the 10-year horizon, and satisfactory benefit-cost ratios. The EOC values of 1 kWh of power were estimated at R$ 0.1990, R$ 0.1791, and R$ 0.3308 for scenarios 1, 2, and 3, respectively, whereas the mean total cost (TC) was R$ 0.5618 (±0.21) considering all scenarios, above the purchase values at CEMIG, which would be R$ 0.50. The TC value was R$ 99,804.42 for scenarios 1 and 2, and R$ 92,424.09 for scenario 3, with a minimum acceptable rate of return of 8.50, while the TOC values were R$ 69,486.62 in scenarios 1 and 2, and R$ 62,229.66 in scenario 3. The amount of power generated in all scenarios was higher than the break-even point. All Monte Carlo simulation models showed positive NPV values, indicating that there is a high probability of being above expectations.