Olive pomace (OP) is the main by‐product of olive oil extraction. After pit and skin removal, OP pulp has high concentrations of dietary fiber and phenolics with high antioxidant capacity. This study evaluated mice health benefits of drum‐dried pitted OP pulp obtained after first and second oil extraction. Fresh OP was steam blanched, then pits and skins separated in a pulper/finisher, and pulp drum‐dried and milled. OP was characterized by proximate analysis, total soluble phenolics (TSP), individual phenolics, and dietary fiber. Drum‐dried pitted OP from first and second extraction was formulated at 10% and 20% in a high fat mice diet. Low fat (5%) and high fat (18%) control diets were also used for comparison. First extraction OP had higher TSP than OP from second extraction. Hydroxytyrosol was the main phenolic in OP. Mice weight gain was lower for the four OP diets compared to high and low‐fat control diets. Fecal protein was high for all OP diets, indicating poor protein retention in mice, possibly by phenolics binding of protein and enzymes. Liver weight and adipose tissue were lower in mice consuming the four high fat OP diets compared to high fat control diet. Also, there was no effect on blood glucose by OP in diets. Mice gut microbiota analysis indicated that Actinobacteria decreased in the OP diets compared to the two control diets while Bacteroidetes increased, indicating a positive correlation with reduced body fat and weight. Drum‐dried pitted OP is a novel agricultural by‐product with its bioactive compounds having the potential to be incorporated in feeds and foods providing health benefits. Practical Application Drum‐dried pitted olive pomace can be produced from first or second olive oil extraction byproducts to be used as a shelf‐stable healthy food or feed supplement.

Radiotherapy is a fundamental part of the treatment of pelvic neoplasms. Up to 90% of patients develop gastrointestinal symptoms as a result of acute injury to the small and large intestine, particularly in the mucosa. Radiotherapy leads to atrophy of the intestinal epithelium, acute crypt inflammation, inflammatory infiltration of the epithelium, malabsorption of lactose, and biliary salts as well as alterations in pancreatic enzymes and biliary salts, resulting in the malabsorption syndrome and dysbiosis. The most commonly reported symptoms of pelvic radiation disease include changes in bowel habits (94%), decreased fecal consistency (80%), frequency of bowel movements (74%), bowel urgency (39%), and fecal incontinence (37%). Although nutritional interventions with dietary modifications have been reported to prevent and treat gastrointestinal symptoms, the evidence remains inconclusive.

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The use of organic byproducts, such as brewery spent grain (BSG), a major byproduct of the beer-brewing industry, has increased in recent years. BSG is rich in fiber, proteins, polyphenols, vitamins, and minerals, as all these compounds remain untapped during the brewing process and is considered a potential source of bioactive molecules with potential health benefits. In this study, two BSG types, regular and a fines fraction, were evaluated. Proximate, amino acid, and dietary fiber analyses were performed to compare the characteristics of both BSG types. Protein increased from 19.4% in regular BSG to 26.9% in BSG fines, while total dietary fiber was reduced from 46.4% to 34.4% in BSG fines. An experimental murine study was conducted where both BSG types were incorporated into a high fat diet formulation at a concentration of 40% to determine whether consumption of these byproducts can provide potential health benefits. A decrease in weight, systemic levels of leptin, and liver fat were observed in mice with BSG incorporated in their diets. Changes in the gut microbiome with an increase in Firmicutes and Bacteroidota and a decrease in Actinobacteria phylum were also observed. Our results indicate that dry fractionation is a feasible technology to increase protein content of BSG and that BSG byproducts in diet may have potential health benefits.

BACKGROUNG: Increased intracellular iron metabolism is a hallmark of breast cancer. Curcumin is an iron chelator with suggested anti-proliferative effects in breast cancer cell lines. However, preclinical studies in murine models are required to validate these important benefits AIMS: Therefore, this study was aimed to determine if the iron-chelating properties of curcumin are responsible for its anti-proliferative effect in breast cancer cells and to investigate the translation of this effect to in vivo models METHODS: For in vitro experiments, human MCF-7 and mouse 4T1 breast cancer cells were tested. Cell proliferation was assessed in presence and absence of different concentrations of FAC (ferric ammonium citrate) and curcumin. For in vivo studies, 4T1 cells were implanted into BALB/c mice. After tumor development, animals were divided into four groups (n=5); control, curcumin, optimized curcumin (OC) and chemotherapy group. Tumor volumes were calculated prior and posterior oral gavage treatments. RESULT: Curcumin inhibited cell proliferation in both MCF-7 and 4T1 cell lines in a seemingly iron-dependent manner. FAC addition inhibited the anti-proliferative effect exhibited by curcumin. Moreover, curcumin group showed a significantly decreased in tumor growth; interestingly, treatment with OC supplement induced the opposite effect. CONCLUSION: These results suggest that curcumin may have an important positive impact in breast cancer, due to its iron dependent anti-proliferative properties.