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The purpose of this study was to assess in vitro the efficacy of nickel-titanium K3 rotary files and hand files for removal of gutta-percha and sealer from obturated root canals using either chloroform or chlorhexidine as solvents. Sixty extracted single-rooted bovine teeth with straight, large canals were prepared, obtured and randomly assigned to 3 groups (n=20). The teeth were stored at 37 degrees C for 1 month and then the gutta-percha and sealer were removed using different techniques, as follows. Group I: size 3 Gates-Glidden drills plus size 30 hand K-files and Hedström files and chloroform; Group II: K3 NiTi rotary files and chloroform; and Group III: K3 NiTi rotary files and 2% chlorhexidine gel. Radiographs were taken and scanned and the images were digitized. The total area of the canal and the area with remaining obturation material were measured in millimeters using a computed image analysis system (ImageLab). Data were analyzed statistically by one-way ANOVA and Tukey test at 5% significance level. The groups differed statistically (p<0.05) with respect to the average percentage of remaining gutta-percha and sealer, presenting the following sequence of effectiveness (from most to least effective): Group I (15.48%), Group II (28.42%) and Group III (35.96%). The findings of this study showed that, despite the technique used for removal of filling material, none of the retreated canals were completely free of gutta-percha and sealer remnants. The use of stainless steel hand files resulted in a lesser amount of filling debris than the use of nickel-titanium rotary instruments.

Aim To characterize the proteome of 20 root canals in teeth with post‐treatment endodontic disease using mass spectrometry and to correlate the identified proteins with clinical features. Methodology Twenty patients with radiographic evidence of apical periodontitis and need for root canal re‐treatment were selected. Samples from the root canal contents were collected and processed using two‐dimensional capillary nano‐flow liquid chromatography and electrospray ionization tandem mass spectrometry. The acquired spectra were separately searched against specific protein database. The results obtained were analysed using descriptive statistics. Additionally, Pearson's chi‐square test or one‐sided Fisher's exact test, as appropriate, was chosen to examine the null hypothesis that there is no relationship between each clinical feature and the presence of specific microbial or human proteins. Significance levels were set at 5% (P < 0.05). Results A total of 1153 human and 720 microbial UniProt accession numbers corresponding to proteins were recovered. The greater prevalence of proteins was related to biological functions, such as cellular and metabolic processes. A considerable number of microbial proteins with clinical relevance functions, such as pathogenesis/virulence, proteolysis, cell adhesion and drug resistance, were detected. Common endodontic pathogens related to post‐treatment endodontic disease such as Enterococcus spp., Propionibacterium spp. and Streptococcus spp. were associated with 23, 40 and 94 distinct proteins, respectively. As for human proteins, many factors related to the immune system process were detected. No significant correlations were found between microbial and human proteins and the clinical features investigated (P > 0.05). Conclusions A considerable number of microbial and human proteins were identified using proteomic analyses, being mainly related to processes indicating cell viability. No significant correlation was found between proteins and clinical features. These findings suggest a network of important microbial pathogenic functions that may be responsible for the host immune system response.

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Objective: This study sought to investigate the prevalence of eight oral Treponemas (Treponema denticola, T. amylovorum, T. maltophilum, T. medium, T. pectinovorum, T. socranskii, T. vicentii and T. lecithinolyticum) in teeth with endodontic treatment failure and periapical lesion.Methods: Samples were taken from 40 root canals presenting endodontic failure and periapical lesion. DNA extraction was performed and Nested-PCR technique was used for the detection of Treponema species using specific primers.Results: Treponemas was detected in 56.5% of the samples analyzed (22/39). Individual root canals yielded a maximum of 6 target Treponema species. T. denticola (30.8%) and T. maltophilum (30.8%) were the most frequently detected species followed by T. medium (20.5%), T. socranskii (20.5%), T. pectinovorum (17.9%) and T. vicentii (17.9%). Positive association was verified between T. denticola and T. maltophilum such as T. medium (P<.05). T. lecithinolyticum was positively associated with intraradicular post (P<.05).Conclusion: The present study revealed that a wide variety of Treponema species plays a role in persistent/secondary infection turning the root canal microbiota even more complex than previously described by endodontic literature. (Eur J Dent 2013;7:61-68)

Cimentos biocerâmicos vêem se tornando populares na ndodontia como material obturador do sistema de canais radicular devido às suas várias vantagens. Objetivo: Revisar a literatura atualmente disponível sobre as propriedades de cimentos biocerâmicos na endodontia. Metodologia: Uma pesquisa bibliográfica exploratória foi realizada utilizando as bases de dados eletrônicas Public Medline (PubMed), Scopus, Embase, e Web of Science empregando o termo de busca “Bioceramic” AND “Endodontic”. Os critérios de inclusão foram: artigos de pesquisas originais e ensaios clínicos publicados em português, espanhol e inglês. Não foram aplicados limites a data de publicação. Resultados: Foram identificados 30 estudos que abordaram propriedades de cimentos biocerâmicos em endodontia publicados em um período de 7 anos (2011-2017). As propriedades e características vantajosas destacadas nos estudos foram: biocompatibilidade, citotoxicidade, bioatividade, capacidade de manchamento dentário, radiopacidade, pH e extravasamento (extravasamento não se enquadra como propriedade), adaptação marginal, resistência de união, resistência a fratura das raízes, capacidade seladora, e propriedades antibacterianas. Conclusão: Os estudos selecionados para esta revisão de literatura mostram que os cimentos endodônticos biocerâmicos apresentam propriedades promissoras para serem utilizados no tratamento de canais radiculares.

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Introdução: o tratamento endodôntico objetiva limpar e modelar o sistema de canais radiculares. O preparo químico-mecânico e a obturação tridimensional (3D) do sistema de canais radiculares são baseados no conhecimento da anatomia dentária interna. As radiografias convencionais e periapicais digitais apresentam limitações na definição da imagem, devido à anatomia 3D do elemento a ser tratado, que é compactada em uma imagem bidimensional. A tomografia computadorizada de feixe cônico (TCFC) é, atualmente, um interessante instrumento para análises anatômicas, devido à precisão volumétrica e ausência de sobreposição de estruturas. Métodos: foram analisados, in vitro, sessenta e quatro (n = 64) primeiros pré-molares inferiores humanos extraídos por indicação terapêutica. Em seguida, foram feitos protótipos com gengiva artificial de silicone, posicionando-se os elementos de forma semelhante a arcadas, para serem geradas as imagens de TCFC e analisadas por um endodontista experiente. O número de canais por raiz foi quantificado em cada elemento dentário e suas configurações anatômicas foram descritas seguindo a classificação de Vertucci. Resultados: foram encontrados 59% de canais Tipo I (n = 38); 0% do Tipo II (n = 0); 9% do Tipo III (n = 6); 6% do Tipo IV (n = 4); 16% do Tipo V (n = 10); 3% do Tipo VI (n = 2); 5% do Tipo VII (n = 3); 0% do Tipo VIII (n = 0) e 2% do Tipo C-shaped. Conclusão: as imagens de TCFC revelam em 3D a anatomia dentária interna, permitindo o planejamento da terapia endodôntica e um mapeamento detalhado da morfologia interna. O conhecimento dessa microanatomia viabiliza uma maior previsibilidade na realização do tratamento, assim como no seu prognóstico, e possibilita melhorar a descontaminação de áreas anatômicas complexas.