Mechanosensitive cation channels and Ca2+ influx through these channels play an important role in regulating endothelial cell functions. TRPC6 is a diacylglycerol-sensitive non-selective cation channel that forms receptor-operated Ca2+ channels in a variety of cell types. Piezo1 is a mechanosensitive cation channel activated by membrane stretch and shear stress in lung endothelial cells. In this study, we report that TRPC6 and Piezo1 channels both contribute to membrane stretch-mediated cation currents and Ca2+ influx or increase in cytosolic free Ca2+ concentration ([Ca2+]cyt) in human pulmonary arterial endothelial cells (PAECs). The membrane stretch-mediated cation currents and increase in [Ca2+]cyt in human PAECs were significantly decreased by GsMTX4, a blocker of Piezo1 channels and by BI-749327, a selective blocker of TRPC6 channels. Extracellular application of OAG, a membrane permeable analogue of diacylglycerol, rapidly induced whole-cell cation currents and increased [Ca2+]cyt in human PAECs and HEK-cells transiently transfected with the human TRPC6 gene. Furthermore, membrane stretch with hypoosmotic or hypotonic solution enhances the cation currents in TRPC6-transfected HEK cells. In HEK cells transfected with the Piezo1 gene, however, OAG had little effect on the cation currents, but membrane stretch significantly enhanced the cation currents. These data indicate that, while both TRPC6 and Piezo1 are involved in generating mechanosensitive cation currents and increases in [Ca2+]cyt in human PAECs undergoing mechanical stimulation, only TRPC6 (but not Piezo1) is sensitive to the second messenger diacylglycerol. Selective blockers of these channels may help develop novel therapies for mechanotransduction-associated pulmonary vascular remodeling in patients with pulmonary arterial hypertension.

Objective:To assess the efficacy and safety of the Wenyang Huoxue method for patients with diabetic peripheral neuropathy.Methods:A systematic literature search was performed using 7 databases: PUBMED, EMBASE, the Chinese National Knowledge Infrastructure, Wanfang, Chinese BioMedical, and the VIP Chinese Science and Technique Journals. The publication time was from the start of each database up to November 2018. Review Manager 5.3 software was used for assessing potential bias, data synthesis, and the subgroup analysis. Begg and Egger tests were used to assess funnel plot symmetries using Stata 14.0 software. The Grading of Recommendations Assessment, Development and Evaluation (GRADE) system was used to assess the quality of evidence.Results:A total of 22 trials involving 1835 participants were eligible. There were significant differences in a total effective rate between the Wenyang Huoxue method combined with Western medicine and Western medicine alone (RR = 1.33, 95% CI 1.26–1.41; P < .00001). As for the sensory conduction velocity (SCV) of the peroneal nerve, the Wenyang Huoxue method combined with Western medicine compared with Western medicine alone had a significant increase (weighted mean difference [WMD] = 5.00, 95% CI 3.42–6.57; P < .00001). Also, the Wenyang Huoxue method combined with Western medicine had significant increases in motor conduction velocity (MCV) of the peroneal nerve (WMD = 4.48, 95% CI 3.78–5.19; P < .00001), tibial nerve SCV (WMD = 3.47, 95% CI 2.66–4.28; P < .00001), tibial nerve MCV (4.87, 95% CI 3.21–6.53; P < .00001), median nerve SCV (WMD = 3.78, 95% CI 3.07–4.50; P < .00001), and median nerve MCV (WMD = 4.50, 95% CI 3.40–5.59; P < .00001). However, the effect of the Wenyang Huoxue method on fasting blood glucose, 2-h postprandial blood glucose, and glycosylated hemoglobin was not statistically significant. Egger's test results showed that there was no publication bias (P = .0008), but the trim and filling method showed steady results. An influence analysis showed that no single study affected the overall result. The GRADE quality of the evidence was low to moderate across the different outcomes.Conclusion:Despite of the apparently positive findings, the quality of GRADE is not high, suggesting that the Wenyang Huoxue method can improve nerve conduction velocity to a certain extent, but more rigorous literature is needed to support this evidence.

BackgroundA significant public health emergency has appeared worldwide since the beginning of 2020. The spread of negative information about COVID-19 on social media poses a challenge and threat to public health disposition and the credibility of government public opinion.ObjectiveThis study aimed to analyze the rules and characteristics of government media in disseminating information on public emergencies. In addition, find ways and means to improve government media's communication power and credibility.MethodBased on relevant theories and measures of information econometrics, 10 WeChat official accounts of the Chinese government were taken as examples. The Python crawler tool was used to collect data of 10 WeChat official accounts-related tweets. In addition, this study used various tools, such as ROST, UCINET, and SPSS, for statistical analysis and co-word analysis of the data.ResultFrom January 17 to March 31, 2020, 6,612 COVID-19-related tweets were published by 10 WeChat official accounts, which broadcast epidemic overview, epidemic prevention and control, science and disinformation, epidemic assistance, epidemic impact, and negative impact. By analyzing the posting time and content of the tweets, we found that changes in the number of articles posted by the WeChat and changes in content and the progress of the COVID-19 pandemic are nearly synchronized, and most tweets are published at 8:00 am. Furthermore, based on the analytics of high dissemination index and high-frequency words, we propose that there is a significant correlation between the strength of independence and the credibility of the WeChat official account.ConclusionThe three elements of WeChat communication (value, interest, and moving) and the degree of independent innovation of public numbers impact the communication power and credibility of government media. First, if the articles published by the WeChat official account are valuable, interesting, and moving, the communication power of the WeChat official account would get more powerful. Second, increased ability for independent innovation has a positive impact on enhancing the WeChat official account's credibility. Third, government media can improve its governance effects of public health emergencies by enhancing their communication power and credibility.

Objective: Currently, it is unclear whether the salviae miltiorrhizae (Danshen Salvia) and ligustrazine hydrochloride (Chuanxiong Chuanxiong) (SMLH) injection combined with mecobalamin can improve diabetic peripheral neuropathy (DPN). We conducted a systematic analysis to evaluate the clinical effects of SMLH injection combined with mecobalamin for treating DPN. Methods: Seven databases, including PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure (CNKI), Wan Fang Database (Wang Fang), Chinese Biomedical Literature Database (CBM), and VIP Database for Chinese Technical Periodicals (VIP) were searched for systematic literature retrieval. Each database was searched up to 2020 to identify randomized controlled trials on DPN treated with SMLH injection combined with mecobalamin. We used the RevMan 5.3 and Stata 14.0 software to assess the risk of bias in the included trials. Results: A total of 15 publications, including 1349 samples, were reviewed. The total effective rate of SMLH injection combined with mecobalamin was 31% higher than that of mecobalamin alone (95% confidence interval [CI] = 1.23–1.38; P < .00001). The experimental group showed a significant increase in the motor conduction velocity (MCV) of the peroneal nerve (weighted mean difference [WMD] = 4.81, 95% CI 3.53–6.09; P < .00001). In addition, SMLH injection combined with mecobalamin showed a statistical significant effect on the sensory conduction velocity (SCV) of the peroneal nerve (WMD = 5.03, 95% CI = 4.16–5.90; P < .00001), and MCV of the median nerve (WMD = 5.38, 95% CI = 4.05–6.72; P < .00001). The WMD for the change in SCV in the median nerve was 4.89 m/s (95% CI = 3.88–5.89; P < .00001). The P-values of the Egger and Begg tests were 0.967 and 0.961, respectively, indicating no publication bias. Subgroup and sensitivity analyses indicated that the results for MCV and SCV of the peroneal nerve and the median nerve were stable. Conclusion: SMLH injection combined with mecobalamin can improve DPN, compared with mecobalamin alone.