Cholesterol oxidase (ChOx) physisorbed onto NiO nanoparticles (nNiO, 22 nm)— chitosan (CHIT) film prepared using coprecipitation method has been characterized using x-ray diffraction, Fourier transform infrared, scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy techniques. The results of electrochemical response studies conducted on ChOx/nan-NiO-CHIT/ITO bioelectrode show linearity of 10–400 mg/dl, detection limit of 43.4 mg/dl, sensitivity of 0.808 μA/(mg dl cm2), fast response time of 15 s, and shelf-life of about 10 weeks. The low value of Michaelis–Menten constant (Km) obtained as 0.67 mM indicates high affinity of ChOx toward the substrate.
The self-assembled ring like nickel (RnNi ∼ 10-20 nm) nanoparticles have been prepared by pulsed laser ablation method and confirmed by transmission electron microscopy. These RnNi nanoparticles electrophoretically deposited onto the indium-tin-oxide (ITO) glass substrate have been functionalized with dimethyl sulfoxide (DMSO) for covalent immobilization of anti-aflatoxin (a-AfB1) monoclonal antibodies and bovine serum albumin as blocking agent. The electrochemical response studies of a-AfB1/DMSO/RnNi-film/ITO bioelectrode reveal linearity as 5-100 ngdL−1, detection limit of 32.7 ngdL−1, sensitivity of 0.59 μA/ng dL−1, and shelf-life of 60 days. The low value (1.3 × 1014 molL−1) of association constant (Ka) shows high affinity towards aflatoxin.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.