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The step-by-step redox process of bis-[2-phenyl-1,2-benzisoselenazol-3(2H)-one-7-yl]diazene i.e. azo-bis-ebselen in the presence of PhSH and H2O2 was reported by using 77Se{1H} NMR spectroscopy. The synthesis of N-thiophenyl ebselenamines as well as...

Novel N-methylated ebselenamine antioxidants were prepared from their corresponding diselenides with iodomethane. All ebselenamines showed excellent chain-breaking and glutathione peroxidase (GPx)-like activities. These could also inhibit the lipid peroxidation much...

Selenazolonamines 10 a-d with free amine (-NH 2 ) group, a new series of preventive and radical-trapping antioxidants were synthesized from their corresponding diselenides. The radical-trapping antioxidant capacity of compounds 10 a-d was determined in a chlorobenzene/ water membrane-like model system in which azo-initiated peroxidation of linoleic acid was carried out in the lipid phase. The regeneration occurred in the aqueous ascorbic acid as co-antioxidant, which could regenerate the antioxidants and enhance their inhibition times. The best antioxidant 10 d substituted with para-NMe 2 a strong electron-donating group quenched the peroxyl radicals more efficiently than α-tocopherol almost 5.5-fold longer inhibition time. The novel selenazolonamines 10 a-d and their derivatives exhibited good preventive i. e. glutathione peroxidaselike activity in the coupled reductase assay. Based on 77 Se NMR spectroscopy, a catalytic cycle for 10 d, involving diselenide, selenosulfide, and selenol as intermediates was proposed. The mechanism for quenching of peroxyl radicals for 10 d was also considered to be involved via protoncoupled electron transfer reaction.

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Background: Keeping in view the importance of natural plant products, bioactivity-guided isolation of phytoconstituents was attempted from Lawsonia inermis L. Materials and Methods: Various polar and non-polar extract/fractions were isolated and evaluated for the genotoxic and antigenotoxic potential against mutagens viz., 4-nitroquinoline-1-oxide and nitrofurantoin in SOS chromotest using Escherichia coli PQ37 tester strain and against H 2 O 2 in DNA protection assay. In order to decipher mode of action, antioxidant activity was evaluated using different assays. The extract/fractions were also investigated for their antiproliferative potential against PC-3 and Colo 205 cancer cell lines. Major polyphenolic constituents were identified using high-performance liquid chromatography (HPLC) technique. Results: All the extract/fractions except Hex-Li significantly decreased the SOS-inducing potency of both the mutagens. In various in vitro assays, the polar fractions exhibited marked antioxidant activity. HPLC analysis of the extract/fractions showed that gallic acid, catechin, chlorogenic acid, epicatechin, rutin, ellagic acid, quercetin and kaempferol are the major constituents. The marked antigenotoxic activity of extract/fractions may be attributed to the polyphenolic compounds. The fractions viz., Hex-Li and CHCl 3 -LI were found to be highly cytotoxic against Colo-205 cancer cell line. Conclusions: This is the first report of antigenotoxic and anticancer activities of Lawsonia inermis L. phytoconstituents. The antiradical potency of these extract/fractions may be responsible for the above activities.

The choice to use teeth for age determination is well accepted due to their longevity ability of being resilient to change. The total of 228 extracted teeth collected from the patients visited to the Department of Oral and Maxillofacial Surgery, NIMS Dental College, Jaipur, the teeth were without dental fillings and without and/or cavity selected. The known age was from 21 to 70 years with the average age of 43.46 years. For age estimation the method according to Gustafson was used. Every tooth was subject to longitudinal section of the midpulpal area. The following dental parameters were studied in each case: attrition, periodontal bone loss, root translucency, secondary dentin deposition, cementum apposition and root resorption. Total scores of different parameters plotted against the chronological age and regression formula was obtained. Using this formula ages were estimated, Gustafson formula was also applied in the same scores and ages estimated. The results of the chronological and estimated age by both formulae have been statistically compared using Pearson's correlation and regression analysis. The results showed strong correlation (0.92; p<0.001) between chronological and estimated age by using both formulae. We found the mean error of ±5.47 by using newly derived and formula and ±6.35 by Gustafson's formula. As a result of our study it was found that newly derived formula provides better results in comparison with Gustafson's formula in Indian population. A positive correlation between age and total scores of physiological changes also revealed.