No abstract

A feeding trial of 60 days was conducted to delineate the effect of dietary synbiotic on maximum growth, body composition, digestive enzyme activity and subsequently gut microbiota in Cirrhinus mrigala fingerlings. One hundred and eighty acclimatized fingerlings of mrigal with initial body weight ranging from 2.87 ± 0.01 g to 3.26 ± 0.05 g were randomly distributed in three replicates of each of four experimental groups including control (without probiotic and prebiotic), T 1 (high probiotic + low prebiotic), T 2 (low probiotic + high prebiotic) and T 3 (high probiotic + high prebiotic), using completely randomized design (CRD). Results showed that growth performance parameters, such as specific growth rate (SGR), per cent weight gain, feed conversion ratio (FCR) and protein efficiency ratio (PER), were reported to be higher in the T 2 group followed by the T 3 group. Maximum gut microbiota activity was found in the T 3 group which was significantly different from other treatment groups. Similarly, body composition and digestive enzyme activity varied significantly (p < .05) among the treatment groups. The study showed the possibility of improved nutrient utilization in terms of growth performance and digestive enzyme activity in the group following dietary synbiotic supplementation. K E Y W O R D Sdigestive enzyme, gut microbiota, prebiotic, probiotic

No abstract

No abstract

Transportation of fish seed is a complex phenomenon associated with multiple kinds of stressors that simultaneously affect the fish in a confined environment, causing stress and mortality. The present study investigated the stress-relieving effect of exogenous glucose as a water additive in different concentrations (0.1, 0.2, 0.3, and 0.4%) during simulated transportation (12 h) of L. rohita fingerlings. The integrated biomarker response (IBR) index is a holistic tool to determine the optimum dose of exogenous glucose for mitigating transportation stress in fish. Based on selected biomarkers related to the stress hormone, serum biochemistry, oxidative stress, and HSP70 mRNA expression, the IBR index is calculated for each treatment and control group. The result showed a significant change in the level of stress hormone cortisol, enzymes (SGPT, LDH, MDH, SOD, CAT) and metabolites (serum glucose, triglyceride, creatinine) along with an upregulation in liver HSP70 mRNA expression. IBR index suggests that 0.2% glucose exhibited the lowest multi-biomarker stress response in comparison to other treatments and control. Therefore, the use of 0.2% glucose as a water additive will provide a solution to transportation induced stress in L. rohita fingerling and will underwrite the success of grow-out fish culture in days to come.

No abstract

Castor protein isolate (CPI) was prepared by aqueous solubilization of defatted castor kernel meal under alkaline conditions (pH 12.0), with precipitation at the isoelectric point (pH 5.0) followed by heat treatment. Five experimental diets were prepared by substituting soybean protein isolate (SPI) at 0%, 25%, 50%, 75%, and 100% with CPI based on protein equivalent basis. The diets were assigned as control, PI25, PI50, PI75, and PI100, respectively. A 60‐day feeding trial was conducted following a completely randomized design. Results revealed significant improvement in growth rates, feed conversion, and protein retention in Labeo rohita fingerlings (P < 0.05) at 124.50 g CPI kg−1 diet (PI75). However, these were similar between control and PI100 (P > 0.05). Dietary CPI did not alter the hepatosomatic index, viscerosomatic index, survival, carcass composition (except crude protein), and digestive enzymes (P > 0.05). The metabolic enzymatic profile of rohu fingerlings was significantly affected due to dietary treatments (P < 0.05) but oxidative stress enzymes were not affected by feeding of CPI. The activity of the aminotransferases, lactate dehydrogenase, and malate dehydrogenase did not differ (P > 0.05) between control and PI100. The preparation of protein isolate is therefore a way forward for the effective valorization of toxic castor meal and could be used at 164.0 g kg−1 inclusion (complete substitution of soybean protein isolate) in the diet of L. rohita. © 2021 Society of Industrial Chemistry and John Wiley & Sons Ltd.

No abstract