No abstract

During the years 2010–2018, avipoxvirus (APV) outbreaks were observed in the domestic chickens and pigeons present in the eastern Indian state of Odisha. Based on typical pox lesions, followed by molecular techniques, the overall morbidity was found to be 18%–19.23% and 16.92%–23% in chickens and pigeons, respectively. The cutaneous forms of the disease were observed with varied rates of mortality, being 47.36%–52.77% in chickens and 39.13%–92% in pigeons. PCR amplification targeting the viral P4b core protein‐coding gene and the DNA polymerase gene confirmed the presence of APV strains in 10 birds. Subsequent phylogenetic analysis of these two genes confirmed that the circulating strains were members of APV clade A. The subclade analysis revealed the introduction of A1 and A3 subclades in Indian chickens and pigeons, respectively. This study is the first molecular record of APVs circulating in eastern Indian birds (Odisha) and involves the first use of the polymerase gene to reveal the circulating clades of Indian APVs.

No abstract

Aim:The objective of this study was to examine the carrier status of theileriosis among apparently healthy cross-bred jersey cattle population of Odisha using conventional blood smear examination and polymerase chain reaction (PCR).Materials and Methods:A total of 34 apparently healthy cross-bred Jersey lactating cows were considered in this study. Blood samples were subjected to microscopic examination after staining with Giemsa stain and PCR based molecular diagnosis using two sets of primer, i.e., N516/N517 and TorF1/TorF2 specific for Theileria annulata and Theileria orientalis, respectively.Results:Examination of blood samples revealed presence of theileria parasites to a magnitude of 20.59% for T. annulata, 8.82% for T. orientalis, and 2.94% for both.Conclusion:Molecular diagnosis was found to be much more sensitive than conventional method for diagnosis of theileriosis. T. annulata was found to be the predominant species affecting the exotic cattle. T. orientalis was detected in apparently healthy cows.

Rice is the most essential source of calories for humans among the cereals and over half of the world's population is fed on rice. As part of a complete food systems approach, biofortification is an effective technique for nutrition enrichment which refers to the development of micronutrient-rich diet by utilising traditional breeding practises and sophisticated biotechnological tools. To enhance the profile of rice grain for biofortification-related properties, researchers must first understand the genetics of critical biofortification characteristics. Significant increases in micronutrients like iron and zinc, as well as many other important minerals and provitamins are acquired in rice grain using the biofortification strategies. Most indica and japonica rice types have been biofortified over the world, giving them the titles of high iron rice, low phytate rice, high zinc rice, and high carotenoid rice or golden rice. Some of the recent approaches towards rice biofortification, as well as their effects, have been explored in this article.

No abstract

Background: Mungbean is one of the most important legume crop with high nutritional value and is consumed in various forms in different parts of India. In order to meet its growing demand, there is a need to increase the yield through adoption of breeding approaches like heterosis breeding and breeding for high yielding varieties. This demands a critical study of the gene action involved in regulation of yield and yield attributing traits which can be achieved through generation mean analysis. Methods: In the present study, generation mean analysis was undertaken using five parameter model to estimate the nature and magnitude of gene action of yield and its component traits in six crosses of greengram. Result: Magnitude of dominance gene effect was reported to be higher than additive gene effect in most of the crosses. Either one or both the interaction components were found significant for all the traits besides number of branches per plant and hundred seed weight. It was evident from the study that the yield components could be improved by exploiting both additive and non-additive gene effects. The transgressive segregants thus produced will prompt the development of desirable high yielding genotypes.

No abstract