The objective of the study was to reduce the dental plaque formation by the inhibitory effect of the extracts taken from Psidium guajava and eggshell against the pathogens present in the plaque. 10 plaque samples were collected and bacterial colony was isolated by using different media (Blood agar media, Nutrient media, and nutrient broth). The Streptococcus mutans have been the major dental plaque pathogen confirmed by 16srRNA sequencing. The inhibition of the Streptococcus mutans (HS SRM) by Psidium guajava and egg shell is proved by antibacterial activity test by antibiotic sensitivity test. Further, GC-MS determined the components of P.guajava which contains Tetrakis (2, 3-Ditert-Butylphenyl), 2, 6, 10, 14, 18, 22-Tetracosahexane, 1, 2-Benzendicarboxylic acid. Egg shell contains Oleic acid, Propyl ester, Squalene, 1, 2-Benzendicarboxylic acid, Pentadecanol. From this current research it has been proven that the Psidium guajava and egg shell is the effective curative agent against dental plaque by inhibiting Streptococcus mutans.
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