The present study was investigated the effects of Ethylenediaminet etraacetic acid(EDTA) contaminated food manipulation on the fertility of male mice the investigtion based on evaluation of physical semencharacteristics, andmorphometrical andhistopathological studies. ofForty healthy adult male Swiss Albino mice have been divided into four groups. The first group (the control) received only standard diet while the second to the fourth groups were supplemented daily with contaminated food with different EDTA concentrations, 0.5, 1.5 and 5 g/kg of food. The samples were collected after two experimental periods (four and eight weeks). Results showed that the EDTA manipulated groups exhibited significantlydecrease of sperm parameters and testosterone concentration. Morphometric study of testis in different EDTA manipulated groups showed a significant decrease of primary spermatocytes and fertile seminiferous tubules in count as compared to control group. However, histological results showed decrement of spermatogeniclineage;declined spermatozoa formation and several necrotic areas. Also, some Sertoli cells and interstitial cells were degenerated. In addition, several blood vessels revealed dilation and congestion in between the interstitial cells. All the adverse alteration in the present results was proportional with the dose of EDTA manipulate on of mice and with the experimental period expansion. In conclusion, the adverse effects of EDTA as a chelating agent induced obviousinhibition of spermatogenic lineage and degeneration of interstitial cells leading to decrease the fertility of male mice.
The present study was conducted to elucidate and compare the adaptive renal structures of freshwater, Bagrus bayad, brackish water Tilapia zilli and seawater Scomber japonicus. The trunk kidneys of the selected fish species were investigated morphometrically, histologically and ultrastructurally. The recorded results showed that the nephrons of Bagrus bayad and Tilapia zilli were distributed within the renal tissue as clusters, while those of Scomber japonicus were scattered solitary within the renal tissue. Bagrus bayad displayed abundant of well vascularized large glomeruli along with thin filtration barrier and numerous wide filtration slits as well as numerous mitochondria of both proximal and distal tubules. However, Scomber japonicus exhibited few number of small glomeruli along with abundance mesangial cells, extremely narrow filtration slits and relatively thick filtration barrier as well as unique giant mitochondria. The structural organizations of the Tilapia zilli nephrons were inter-between Bagrus bayad and Scomber japonicus. As a whole, the results of the current study showed that the kidney of the examined fish species exhibited specific adaptive renal structural features to fulfill their variable osmoregulatory demands.
Objective: To identify polymorphism of Factor V Leiden and Prothrombin gene in women suffering from preeclampsia. Study design: From 142 pregnant women we identified 92 women suffering from preeclampsia and 50 healthy controls with normal pregnancy matched for age and socioeconomic status, preeclampsic patient classified as mild preeclampsia 42(45.7%) and severe preeclampsia 50(54.3%). Blood samples were tested for DNA polymorphism affecting thrombophilia Factor V Leiden and Prothrombin gene polymorphism. Results: Heterozygous AG genotype showed a significant high frequency among preeclampsic patients (20.7%) compared to controls (4.0%), (OR 6.2, P= 0.006) regarding to Prothrombin gene but: Factor V Leiden, AG genotype showed (8.7%) of preeclampsic patients which was absent in any of the controls.
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