Evaluation of the Effect of Edta Contaminated Food on the Fertility of Male Mice

Abstract

The present study was investigated the effects of Ethylenediaminet etraacetic acid(EDTA) contaminated food manipulation on the fertility of male mice the investigtion based on evaluation of physical semencharacteristics, andmorphometrical andhistopathological studies. ofForty healthy adult male Swiss Albino mice have been divided into four groups. The first group (the control) received only standard diet while the second to the fourth groups were supplemented daily with contaminated food with different EDTA concentrations, 0.5, 1.5 and 5 g/kg of food. The samples were collected after two experimental periods (four and eight weeks). Results showed that the EDTA manipulated groups exhibited significantlydecrease of sperm parameters and testosterone concentration. Morphometric study of testis in different EDTA manipulated groups showed a significant decrease of primary spermatocytes and fertile seminiferous tubules in count as compared to control group. However, histological results showed decrement of spermatogeniclineage;declined spermatozoa formation and several necrotic areas. Also, some Sertoli cells and interstitial cells were degenerated. In addition, several blood vessels revealed dilation and congestion in between the interstitial cells. All the adverse alteration in the present results was proportional with the dose of EDTA manipulate on of mice and with the experimental period expansion. In conclusion, the adverse effects of EDTA as a chelating agent induced obviousinhibition of spermatogenic lineage and degeneration of interstitial cells leading to decrease the fertility of male mice.

Toxicity of bean cooking media containing EDTA in mice

“…However, prolonged oral administration of EDTA could be toxic. EDTA toxicity depends on the doses and the route of administration (Ramadan et al, 2016). Therefore, two sublethal doses of EDTA were tried: 20 mg/kg and 200 mg/kg, which correspond to 1/100th and 1/10th of LD 50 , respectively.…”

“…In experimental animals, EDTA administration promoted histological alterations (Khalil et al, 2008; Ramadan et al, 2016; Wang et al, 2004). The present study indicated that there were prominent histopathological alterations in the liver of mice administered EDTA alone or in the bean cooking media.…”

“…Abdel-Wahhab et al (2002) reported that EDTA administration showed kidney dysfunction. Furthermore, Ramadan et al (2016) reported that having food contaminated with EDTA caused degeneration in the interstitial cells in mice and decreased the fertility.…”

“…Hagiwara et al (2006) reported that EDTA administration increased the levels of chromosome aberrations in Syrian hamster embryo (SHE) cells. Recently, Ramadan et al (2016) reported that feeding male mice on diet containing EDTA (0.5–5 g/kg of food) decreased their fertility in dose-dependent manner. In previous studies, administration of EDTA orally produced reproductive and developmental adverse effects in animals (Khalil et al, 2008; Lanigan and Yamarik, 2002).…”

Effect of sub-acute and sub-chronic administration of Vicia faba's cooking medium containing EDTA on hepato-renal functions in albino mice

“…Prolonged oral administration of EDTA could be toxic depending on the administration route and the doses (Ramadan et al 2016). Previous studies reported that cooking could alter phytochemicals constitute and hence in uence the bioactive compounds and antioxidant capacity in food (Podsedek et al 2008;Chan et al 2009;Rawson et al 2013;Gunathilake et al 2018).…”