Summary. -Clerodendron inerme, a common hedge plant grown in India, is affected by a yellow mosaic disease caused by a begomovirus. In the present study, the complete genome (DNA A) of this virus was cloned and sequenced.
Grapes downy mildew is incited by the most destructive pathogen called P. viticola is a major bottleneck for grapevine production. The morphological and ultra structural analysis of the pathogen plays a significant role in order to take precautionary management strategies. For morphometric analysis the downy mildew infected leaf samples were collected from eight different locations in Coimbatore and Theni districts. The collected samples were subjected to morphoplogical analysis under light microscope at 40 X magnification. The length and breadth of the sporangia vary from 19.94 -22.73 µm and 14.58-17.69 µm. The scanning electron microscopic analysis of P. viticola shows the developmental stages of the pathogen on the host leaf surface. The Pathogenicity of the pathogen was confirmed through detached leaf assay. The characteristic sporulation of the fungus was observed at 7 th day after post inoculation.
Yellow mosaic disease caused by mungbean yellow mosaic virus (MYMV) belonging to the genus Begomovirus (the family Geminiviridae) is a major constraint in cultivation of grain legumes in India. The urdbean (Vigna mungo (L.) Hepper) and mungbean (Vigna radiata (L.) R. Wilczek) samples affected with yellow mosaic disease exhibits yellow mosaic symptoms along with leaf puckering and leaf distortion in Tamil Nadu. Hence the study was performed to find out if there was any association and influence of betasatellite DNA on the symptom expression of MYMV. Full length viral clones of DNA A and DNA B were obtained through rolling circle amplification from YMD infected samples and identified as mungbean yellow mosaic virus. Interestingly, betasatellite was found to associate with MYMV, and its nucleotide sequence analysis showed its 95% identity with papaya leaf curl betasatellite (DQ118862) from cowpea. The present study represents the first report about the association of papaya leaf curl betasatellite with MYMV and represents a new member of the emerging group of bipartite begomovirus associated with betasatellite DNA.
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