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Shrimp and squid often cause food allergy. In order to prevent malnutrition, allergic patients are advised that they can eat foods that contain allergy-inducing ingredients as long as the intake does not cause an allergic reaction. However, there is no hypoallergenic treatment applicable to invertebrate seafood. Thus, in this study, we aimed to develop a hypoallergenic treatment for invertebrate seafood by removing the major allergen tropomyosin (TM) in invertebrate seafood. When muscle samples of shrimp and squid were subjected to boiling for 10 min, only 11.2 and 35.0% of TM remained in the muscles, respectively. Thus, boiling is considered to be an efficient hypoallergenic method, particularly with respect to shrimp.