Background: gastric cancer (GC) is one of the common cancers with a high mortality rate and complex pathogenesis. In recent years many studies focused on the roles of non-coding RNA in GC biology. Long noncoding RNAs (lnc-RNAs) like HOTAIR and SNHG-7 are a class of non-coding RNA that can affect the cell’s biology by sponging of micro-RNAs. Recent studies reveal the relation between these lnc-RNAs and miR-34a expression which can affect various molecular pathways in malignant cells. Methods: GeneAll Trizol RNA extraction kit (Korea) was used to extract total RNA according to the supplied procedures and gene expression was evaluated. The qRT-PCR assay was performed to evaluate the expression levels of miR-34a, HOTAIR, and SNHG-7 in GC and paired marginal tissue specimens. The AUC of the ROC curve was estimated based on their expression in GC and gastric normal tissues to evaluate their diagnostic accuracy.Results: Expression levels of miR-34a were higher in adjacent marginal samples compared to GC tissue samples. We noted significantly higher levels of HOTAIR expression in GC samples compared to non-tumor adjacent tissue samples and the expression of HOTAIR in GC tissues was negatively correlated with mir-34a. Similarly, we noted GC tissue samples showed higher levels of SNHG-7 expression and that SNHG-7 expression was negatively correlated with mir-34a. Regarding the clinicopathological factors, miR-34a expression was lower in patients with advanced GC, while enhanced expression of HOTAIR and SNHG-7 was noted in these patients. Conclusion: Our findings suggested that miR-34a, HOTAIR, and SNHG-7 expression levels have high potential as diagnostic markers for discriminating GC patients from normal cases. In Addition, there is a negative correlation between miR-34a with two other genes which suggests the regulatory effects of HOTAIR and SNHG-7 on miR-34a.
Introduction: Meconium-stained amniotic fluid (MSAF) may increase maternal and neonatal complications. The favorable mode of delivery is controversial in women with MSAF. This study aimed to compare umbilical cord arterial blood gas (ABG) and neonatal outcomes in deliveries complicated by meconium and clear amniotic fluid during the active phase of labor. Materials and Methods: This observational study was performed on 144 term pregnant women (72 with MSAF and 72 with clear amniotic fluid) during the active phase of labor with ruptured membranes admitted to Al-Zahra and Taleghani hospitals in Tabriz, Iran from June 2019 to June 2020. A checklist was used to record the ABG and neonatal outcomes. Results: The frequency of cesarean delivery in the MSAF group (25%) was significantly higher than in the women with clear amniotic fluid (20%) (P= 0.006). There was no statistically significant difference between the groups regarding pH, base excess, PCO2, 1- and 5- minute Apgar scores, and neonatal intensive care unit (NICU) admission of the newborn due to MSAF (P> 0.05). There was no incidence of hypoxic-ischemic encephalopathy, sepsis, and seizure. Conclusion: Given the non-significant difference between two groups of women with and without MSAF during labor in terms of neonatal outcomes and umbilical cord ABG, it seems that MSAF alone is not an indication for cesarean delivery. Thus, vaginal delivery is recommended in pregnant women with MSAF.
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