A 5-week study was conducted to determine the effects of different stocking density on hematological parameters and growth performance of great sturgeon. Fish were cultured in duplicates of fiberglass tanks under stocking densities of 50, 75, 100 and 125 fish/m3 as T1, T2, T3 and T4 respectively. Based on the results, significant increase (p<0.05) in total body weight and total length were related to low density group. The lowest mean body weight (103.26 g) and length (29.65 cm) were recorded in T4. At the end of the experiment, Results of hematological analysis showed changes in the stocking density had non-significant impact on values of WBC and MCH (p>0.05). The highest mean corpuscular hemoglobin concentration (MCHC) was also recorded in T4. The highest level of hemoglobin, hematocrit (7.25 g dL-1 and 22.2%) were significantly associated with T1 (p<0.05).
A 4 days study was conducted to determine the effects of Different concentration of bioaugmentor bacterial strain Bacillus licheniformisi, B. subtilis, B. polymyxa, B. laterosporus and B. circulans (Protexin Aquatech, UK) on adjustment of parameters of water quality as TAN, NO2-N, NO3-N and turbidity from the effluent of Common Carp ponds. Effects of time and concentration were studied as a completely randomized split-plot design. Time and concentration and their interaction had a significant difference (P<0.05)on changes of total ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and turbidity. The total counts of bacteria recorded in the water of bioremedated tanks were also lower than that in the control tank.
This study was done to evaluate the effect of different quercetin levels on growth performance, immune responses, antioxidant status, serum biochemical factors, and high-temperature stress responses in common carp (Cyprinus carpio). A total number of 216 common carp with an average weight of 27.21 ± 53 g were divided into 12 tanks ( four treatments × three replications ) and fed 0 mg/kg quercetin (T0), 200 mg/kg quercetin (T1), 400 mg/kg quercetin (T2), and 600 mg/kg quercetin (T3) for 60 days. There were significant differences in growth performance, and the highest final body weight (FBW), weight gain (WG), specific growth rate (SGR), and feed intake (FI) were observed in T2 and T3 ( P < 0.05 ). Different quercetin levels significantly increased complement pathway activity (ACH50) and lysozyme activity both before and after heat stress ( P < 0.05 ). Catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) were significantly increased in fish exposed to heat stress, but fish fed with a supplemented diet with quercetin showed the lowest levels both before and after heat stress ( P < 0.05 ). Superoxide dismutase (SOD) levels were significantly enhanced in fish fed diets supplemented with quercetin in both phases ( P < 0.05 ). Different quercetin levels led to a significant decrease in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) before and after the challenging test ( P < 0.05 ). Glucose and cortisol levels were significantly higher in the control group compared to the other treatments in both phases ( P < 0.05 ). The expression of glutathione peroxidase (GPx) and lysozyme was markedly upregulated in fish fed with quercetin-supplemented diets ( P < 0.05 ). No marked effects were observed for growth hormone (GR) and interleukin-8 (IL8) ( P > 0.05 ). In conclusion, dietary quercetin supplementations (400-600 mg/kg quercetin) improved growth performance, immunity, and antioxidant status and increased tolerance to heat stress.
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