In vitro culture has the greatest commercial value in the extremely quick creation of clonal plants compared to conventional techniques of propagation. It has also proven to be of tremendous practical utility as assistance to the development and maintenance of disease-free planting material, germplasm conservation, and supplements to the regular methods of plant progress, such as plant genetic engineering. The discovery and implementation of current tissue culture techniques are thought to be paving the way for a second green revolution. Here, the standardization of techniques for the in vitro clonal propagation of fruit crops is highlighted. In addition, we summarize the effects of several surface sterilizing agents, plant growth regulators (PGRs), and anti-phenolic chemicals on culture establishment, shoot proliferation, in vitro rooting, hardening and economics of in vitro generation of true-to-type plants.
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