Peroxidase, polyphenol oxidase (PPO), phenolic compounds and total sugars (TS) were investigated during root formation in cuttings of Berberis vulgaris var. asperma (BVA) and Berberis thunbergii var. atropurpurea (BTA) treated with indole-3-butyric acid (IBA) and IBA+H2O2. Rooting was observed on BTA cuttings but not on BVA cuttings. The BTA cuttings treated with IBA and IBA+H2O2 showed higher rooting percentages, number of roots, and root length over the control. Those treated with IBA+H2O2 recorded the lowest peroxidase activity after planting. BTA cuttings treated with IBA+H2O2 showed the highest peroxidase activity at 50 d after planting; BVA cuttings under different treatments showed no significant difference for peroxidase activity at planting time or up to 80 d after planting. PPO activity for the BTA cuttings in the control treatment was lower than for other treatments during root formation. The cuttings in the IBA and IBA+H2O2 treatments showed increased PPO activity from 0 to 50 d after planting and a slight decrease in PPO activity from 60 to 80 d after planting. PPO activity for the BVA cuttings was significantly lower than for BTA during root formation. The BTA cuttings treated with IBA and IBA+H2O2 showed the highest phenolic compound content during root formation. The BVA cuttings displayed higher TS than BTA during the initial stage of root formation. A comparison of the anatomical structure of easy-to-root and difficult-to-root cuttings indicated that physical inhibitors did not affect the rooting capacity of BVA.
Reducing iron solubility in calcareous soils causes iron chlorosis symptoms in the plant. In order to investigate the response of M. scutellata to direct and indirect iron deficiency, a factorial experiment was conducted in a completely randomized design with three replications. The experimental factors included three levels of lime (0, 4 and 8%) and five levels of Fe (0, 5 and 10 mg Fe Kg soil-1 as soil application and 0 and 2.1 µM Fe as foliar spray). Zero Fe concentrations in soil and foliar Fe fertilization was considered as controlS and control-F treatment, respectively. The results showed that the amendment of 8% CaCO3 in control treatments caused typical Fe deficiency symptoms, including decreases in chlorophyll, total and extractable leaf Fe concentration, protein, leaf relative water content, membrane stability index and increases in leaf total phenolic and flavonoid concentration when compared to 0% lime. The soil and foliar Fe fertilisation led to the improvement of Fe chlorosis, increases in chlorophyll, Fe, protein, and decreases in concentration of phenolic compounds in three levels of lime. The foliar Fe fertilization in three levels of lime increased total and extractable leaf Fe content, compared to two soil Fe treatments, but foliar Fe fertilization (in 4 and 8% of lime) yielded progressively lower leaf phenolic compounds. The highest leaf chl concentration in 8% lime was observed in 10 mg Fe Kg soil-1 and foliar Fe fertilization. The presented data indicate that the use of foliar application of Fe (Fe-EDDHA) is potent to reduce the effects of lime induced chlorosis in plants in calcareous soils.
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