Micropropagation of endangered Iris ferdowsii Joharchi &amp; Memariani through callus induction

Safari, N; Tehranifar, Ali; Kharrazi, Mahdiyeh

doi:10.1007/s11240-023-02535-1

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…One of the fundamental ideas behind protecting wild plants as a genetic source is selecting the best method of plant propagation (Safari et al 2023). Plant tissue culture uses growth regulators for its growth, development and differentiation.…”

Section: Introductionmentioning

confidence: 99%

A comparative analysis of antioxidant and bioactive constituents in micropropagated and wild Lindernia pusilla medicinal herb

Baro,

Das,

Boro

et al. 2023

Preprint

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Lindernia pusila is one of the important medicinal herbs which is being used to treat various diseases. The present experiment is aimed to develop an efficient protocol for in vitro mass propagation of L. pusila and to study somaclonal variations in the micro propagated plants using RAPD technique. Surface sterilization of collected explants of L. pusila was performed using 0.1% mercuric chloride and cultured in MS media with different concentrations of growth regulators. Explant surface sterilization was most effective at 2 & 3 minutes of treatment with 0.1% mercuric chloride resulting in 75% of the explant survival. The media MS + 1 mg/L BAP + 0.2 mg/L NAA showed maximum shoot proliferation and multiplication per explant. After hardening of tissue cultured plants, the whole genome of wild and micro propagated plants was isolated using the DNeasy Plant Mini Kit and RAPD assay was performed using 14 different RAPD primers. Among the 14 RAPD primers, 7 primers formed polymorphic DNA bands in tissue-cultured Lindernia pusila plants. Comparatively the tissue cultured plant extract showed higher content of phenols, flavonoids, and anti-oxidant properties than the wild extracts of L. pusilla. In Chromatographic study of gallic acid, the tissue cultured extract showed more gallic acid concentration (42.9mg/g) than the wild sample (10.9mg/g) and the quercetin constituent of tissue cultured plant was higher than the wild extract. The volatile bioactive components found in the wild and tissue cultured extracts were studied using GC-MS analysis.

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Section: Introductionmentioning

confidence: 99%

A comparative analysis of antioxidant and bioactive constituents in micropropagated and wild Lindernia pusilla medicinal herb

Baro,

Das,

Boro

et al. 2023

Preprint

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Micropropagation via somatic embryogenesis of Iris pallida Lam. ecotypes

Meucci,

Ghelardi,

Maggini

et al. 2024

Plant Cell Tiss Organ Cult

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Iris pallida LAM., which has characterized the economy of small and medium-sized Tuscan farms for decades, is one of the most typical rustic species of the Mediterranean landscape. I. pallida essence is widely used in the perfumery sector for the indicative smell of violet caused by its high content of irones, ketone compounds accumulated inside the rhizome during their storage phase. One of the main critical aspects of its cultivation is the vegetative propagation method, traditionally carried out by rhizome transplanting, which does not allow obtaining a sufficient number of plants to encourage its cultivation. The state of the art indicates the micropropagation of I. pallida via somatic embryogenesis as the most effective propagation method, using immature flower components as starting tissue; however, there are no studies aimed at comparing the responses of different ecotypes of I. pallida to this technique. In this study, I. pallida clones of HE, VIC and BA ecotypes were obtained via somatic embryogenesis, starting from bud (young and immature) and leaf explants, monitoring all the development steps from callus to plantlets production, and testing clones adaptation to field conditions, using a modified acclimatization protocol. The results highlighted the different responses of the ecotypes to the in vitro protocol and demonstrated the effectiveness of somatic embryogenesis in producing I. pallida plantlets with a chromatographic profile overlapping with the donor plant; this alternative propagation method could allow to produce plantlets without sacrificing sealable rhizome.

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6-Benzylaminopurine mediated indirect organogenesis in Sapindus trifoliatus L. through internodal segments

Asthana,

Rai,

Jaiswal

2024

Vegetos

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Micropropagation of endangered Iris ferdowsii Joharchi & Memariani through callus induction

Cited by 3 publications

References 36 publications

A comparative analysis of antioxidant and bioactive constituents in micropropagated and wild Lindernia pusilla medicinal herb

A comparative analysis of antioxidant and bioactive constituents in micropropagated and wild Lindernia pusilla medicinal herb

Micropropagation via somatic embryogenesis of Iris pallida Lam. ecotypes

6-Benzylaminopurine mediated indirect organogenesis in Sapindus trifoliatus L. through internodal segments

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