AIM: The purpose of this study was to analyze the phenolic content and antioxidant activity of both water and ethanol extracts of a combination of several herbs (Phyllanthus urinaria, Andrographis paniculata, Curcuma longa, Zingiber officinale, Citrus limon, and Cymbopogon citratus), as well as product development in the form of nanoemulsions.
METHODS: The research was conducted by making three combinations of herbs mixtures with various compositions (Formulas A, B, and C). The combined herbs powder was then made in the form of water and ethanol extracts. The ethanol extract of each herbal combination was then made also in the form of a nanoemulsion by spontaneous emulsion.
RESULTS: The ethanol extract of each herbs combination contained higher phenolic compounds and antioxidant activity than the water extract. The nanoemulsion product of the combined herbs ethanol extract had a particle size of 21.1; 34.9; and 50.7 nm and showed high antioxidant activity.
CONCLUSION: The combined several herbs (P. urinaria, A. paniculata, C. longa, Z. officinale, C. limon, and C. citratus) ethanol extract and its nanoemulsion products have the potential to be developed as natural antioxidants.
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