Yeast can be isolated from tofu wastewater and the cell wall in the form of β-glucan can act as a natural decontaminant agent. This study aimed to isolate and characterize native yeast from tofu wastewater, which can be extracted to obtain β-glucan and then identify the yeast and its β-glucan activity regarding antifungal ability against Aspergillus flavus and aflatoxin-reducing activity towards aflatoxin B1 (AFB1) and B2 (AFB2). Tofu wastewater native yeast was molecularly identified, and the growth observed based on optical density for 96 h and the pH also measured. β-glucan was extracted from native yeast cell walls with the acid-base method and then the inhibition activity towards A. flavus was tested using the well diffusion method and microscopic observation. AFB1 and AFB2 reduction were identified using HPLC LC-MS/MS. The results showed that the native yeast isolated was Pichia norvegensis with a β-glucan yield of 6.59%. Pichia norvegensis and its β-glucan showed an inhibition zone against Aspergillus flavus of 11.33 ± 4.93 and 7.33 ± 3.51 mm, respectively. Total aflatoxin-reducing activity was also shown by Pichia norvegensis of 26.85 ± 2.87%, and β-glucan of 27.30 ± 1.49%, while AFB1- and AFB2-reducing activity by Pichia norvegensis was 36.97 ± 3.07% and 27.13 ± 1.69%, and β-glucan was 27.13 ± 1.69% and 32.59 ± 4.20%, respectively.
Tujuan dari penelitian ini adalah untuk mengetahui pengaruh perbandingan rumput laut Eucheuma cottonii dengan sari wortel dan konsentrasi sukrosa terhadap karakteristik marshmallow wortel. Metode penelitian yang dilakukan terdiri atas dua tahap, yaitu penelitian pendahuluan dan penelitian utama. Penelitian pendahuluan yaitu menentukan formula yang paling tepat pada pembuatan marshmallow wortel. Rancangan percobaan yang digunakan dalam penelitian ini adalah rancangan acak kelompok (RAK) dengan pola faktorial 3x3 dan diulang sebanyak 3 kali. Rancangan perlakuan yang dilakukan terdiri dari dua faktor meliputi : perbandingan rumput laut Eucheuma cottonii dengan sari wortel (A) yang terdiri dari 3 taraf, yaitu a1 (1:0,5), a2 (1:1), a3 (1,5:1) dan konsentrasi sukrosa (B) yang terdiri dari 3 taraf, yaitu b1 (25%), b2 (30%), b3 (35%). Hasil penelitian pendahuluan menunjukkan formula 1 yang akan digunakan pada penelitian utama. Hasil penelitian utama menunjukkan perbandingan rumput laut Eucheuma cottonii dengan sari wortel berpengaruh terhadap organoleptik dalam hal warna, aroma, rasa, dan tekstur, serta kadar air. Konsentrasi sukrosa berpengaruh terhadap organoleptik dalam hal warna, aroma, rasa, dan tekstur, serta kadar air dan kadar gula reduksi. Interaksi antara perbandingan rumput laut Eucheuma cottonii dengan sari wortel dan konsentrasi sukrosa berpengaruh terhadap organoleptik dalam hal warna, aroma, rasa, dan tekstur. Berdasarkan respon organoleptik didapatkan produk terpilih yaitu a2b3 (perbandingan rumput laut Eucheuma cottonii dengan sari wortel 1:1 dan konsentrasi sukrosa 35%). Produk terpilih tersebut memiliki kadar air 26,99%, kadar gula reduksi 5,6%, kadar karotenoid 19,54 ppm, nilai uji warna (L* = 54,55 ; a* = 6,60 ; b* = 13,47), kadar vitamin C 0,85 mg/100 g, kadar serat kasar 2,6%, kadar kalsium 14,22 mg/100 g, nilai elastisitas (springiness) 0,614, nilai kekenyalan (chewiness) 2,0622 g.sec dan nilai organoleptik rata-rata suka.
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