A B S T R A C T The development of immune deposits on the subepithelial surface of the glomerular capillary wall was studied in isolated rat kidneys perfused at controlled perfusion pressure, pH, temperature, and flow rates with recirculating oxygenated perfusate containing bovine serum albumin (BSA)

These results indicate that the presence of high grade prostatic intraepithelial neoplasia on needle biopsy is strongly predictive of carcinoma. Prostatic intraepithelial neoplasia should be reported in needle biopsies and biopsy repeated. These finding support the hypothesis that prostatic intraepithelial neoplasia is a precursor of prostate cancer.

Of 46 patients with acute crescentic glomerulonephritis involving 20 to 90% of glomeruli, 16 had no definable systemic disease and no significant glomerular immune deposits by immunofluorescent or electron microscopy. Anti-GBM antibody and circulating immune complexes were further excluded by radioimmunoassay and Raji cell assay in all patients tested. Clinical features included a 10:6 male:female ratio, mean age of 58 years (range, 13-77), disease duration of less than 3 months, rapidly deteriorating renal function, and frequent pulmonary manifestations. Nine patients had oliguria, serum creatinine concentrations over 6 mg/100 ml, and required dialysis, but three of these patients subsequently recovered renal function. These three patients and seven patients with creatinine concentrations of less than 6 mg/100 ml have not progressed to chronic renal failure. In this series, idiopathic acute crescentic glomerulonephritis without immune deposits was more common than was immune complex or anti-GBM nephritis. The clinical, laboratory, and pathologic characteristics of these patients were similar to those reported in anti-GBM and immune-complex-induced glomerulonephritis. These observations expand the spectrum of rapidly progressive crescentic glomerulonephritis. They suggest that glomerular immune deposits may be less important than other factors in determining the extent of renal injury and subsequent clinical course in crescentic glomerulonephritis.

A B S T R A C T The effect of increased capillary perilmealility on1 glomerular immultine comiiplex localizationi was studied in rats immiluniized with proximial tul)ular anitigeni (Fx1A) to induce autologouis imnmunie complex nephropathy (AICN). AICN rats were made proteinuric 1)V-injection or uniilateral renal perfusion with amiiinoinucleoside of puromycin (PA) before developing sul)epithelial conmplex deposits. Control AICN kidneys developed diffuse granular deposits of IgG and Fx1A oIn the subepithelial surf'ace of the glomerular b)asement membrane (GBM) at 3 wk by immuiinofluiorescence and electron microscopy, and dep(isits increased in sul)-se(quienit weekly biopsies. In conitrast, PA-nephrotic AICN kidneys developed few or no GBM deposits and a significant increase in mesangial localization of IgG and FxlA during the period of PA-induced proteinuria. These alterationis in complex localizationi were documenited 1)oth in rats with PA nephrosis andl in unilaterally PA-inephrotic kidneys compared with conitralateral controls in the sanme animiials, thus excluidinig any effect of PA on the imnililiiopatlhogenetic mlechaniisnm in AICN as ani explaniation for these finidings. The absence of GBMi deposits closely correlated with reducedl stainiing for polyanionic glomierilar sialoproteini in proteinutiric kidneys, siniee PA-perfuised kidneys studied 2 wk after resdoltutioin of proteinutria demiion strated retuirni of niormlial staininig for sialoprotein Portionis of this work were presented at the 8th Ainutal