Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinae
sub-family and belongs to genus Rhadinovirus
. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical capsid symmetry surrounded by a loose envelope, typical of a herpesvirus. A 2,571 bp PCR product after Hind
III digestion generated four fragments, whose base pair composition were 403, 420, 535, and 1,125 bp. Restriction enzymes Hind
III and Bam
HI generated the expected diagnostic bands as well as a 2,350 bp hypermolar fragment as a result of Bam
HI treatment to demonstrate that agent was a bovine herpesvirus 4, appertaining to DN-599 group.
ABSTRACT. First record of Aedes (Stegomyia) albopictus (Skuse) (Diptera, Culicidae) in Morretes, coastal plain, Paraná State, Brazil. Specimens of Aedes albopiclus (Skuse, 1894) were callgth lIsing human bait with electric aspirator and a Illoditied COC trapo
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.