The purpose of the research is to characterize Staphylococcus aureus colonization in healthy population of Algiers, to assess the impact on diagnostic performance of systematic additional broth enrichment, and to ascertain the additional benefits of multiple site screening. In order to more accurately determine the prevalence of S. aureus colonization, the swab specimens from multiple screening sites were incubated in brain-heart broth before agar plating. From 2009 to 2011, 1176 samples were collected from 459 participants (201 adults and 258 children). The additional enrichment detection step significantly increased S. aureus detection rates (p < 0.0001). S. aureus nasal detection was positive in 37.8% of adults, and the addition of throat samplings significantly increased the S. aureus detection rate up to 54.7% (p < 0.001). S. aureus nasal detection was positive in 37.6% of children. The addition of throat samplings in children significantly increased the S. aureus detection rate up to 53.1% (p < 0.001) and that of anal samplings up to 59.7%. The overall prevalence of methicillin-resistant S. aureus was 5.2% (3% of adults and 7% of children). spa typing of all isolates revealed a diverse but strongly clonal S. aureus population structure. This approach involving multiple anatomical sampling sites and an additional enrichment of the swabs before conventional culture significantly increases the detection rate of S. aureus carriers and may prove valuable to improve global S. aureus infection prevention.
Background and Aim: Marek's disease (MD) is a lymphoproliferative disease that occurs in chickens. In the absence of control measures, MD causes devastating losses to commercial poultry flocks. Vaccination has enabled dramatic success in the prevention and control of MD. However, the MD vaccination program has failed frequently, and occasional clinical outbreaks have been reported in the vaccinated flocks as well. The present study aimed to describe the clinical and histopathological characteristics of the field cases of MD in broiler breeder flocks.
Materials and Methods: A survey on the update of MD occurrence in Algerian broiler breeder flocks was conducted from June 2020 to September 2020. Ten vaccinated broiler breeder flocks located in Central Algeria and having progressive tumors in different visceral organs were evaluated for MD virus infection by conducting a histopathological examination of the birds.
Results: The age of the birds affected with MD ranged from 13 to 22 weeks. The mortality rate varied sensitively from 4% to 10%. The clinical symptoms reported in the affected flocks included locomotor, nervous, digestive, and respiratory symptoms. Necropsy of the dead or euthanized birds revealed visceral lymphomatosis in several organs and macroscopic changes in the peripheral nerves (including loss of longitudinal striation, color change [grayish], and volume increase). The histopathological findings included the infiltration and proliferation of lymphocytes and blast cells (lymphoblasts) in various organs of the birds, which are the typical characteristics of MD and, therefore, confirmed the field infection of MD in these birds.
Conclusion: The present study provided evidence for the high prevalence of MD in the broiler breeder flocks vaccinated with a bivalent vaccine (turkey herpesvirus+Rispens) at the hatchery. The findings of the present study may indicate highlevel failure of vaccination in these birds.
This study was carried out to determine the association between milk production characteristics at the start of lactation (the first three month of lactation) and the performance of reproduction within dairy cows of Algeria. Data were collected from Holstein cows (n=920) that calved between October 2017 and October 2019. Reproductive parameters included in the study were: calving to first-service interval (CFSI, days), calving to conception interval (CCI, days), services per conception (SPC), and conception rate at first-service (CRFS %). Milk parameters such as peak milk yield, cumulative milk yield in the first 30 days (Milk30), 60 days (Milk60) and 100 days (Milk100) postpartum of the cows. The milk composition traits analyzed were milk fat concentration (MF %) and milk protein concentration (MP %). These variables were used to calculate fat to protein ratio (FPR) for each stage of lactation (stage 1: 15–30 days, stage 2: 45–60 days and stage 3: 75–90 days postpartum). Pearson’s correlation analysis was used to determine the correlation between reproductive parameters and milk production variables.The obtained results showed that peak milk yield, cumulative milk yield in the first 60 days (Milk60) and 100 days (Milk100) were significantly and positively (P<0.05) correlated with all the measured fertility parameters. Furthermore, fat to protein ratio (FPR) and milk fat content correlated significantly and negatively (P<0.05) with CFSI, CCI and SPC in the first two stages. The correlation between milk protein content with CFSI, CCI and SPC were negative and significant in the all three stages. Further research is required to identify the cause of this association.
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