Newly-hatched Epinephelus coioides larvae were stocked in five 5-ton tanks at an initial density of 25,000 ind/tank. Copepod nauplii were propagated in four of these tanks by inoculating various densi ties (20 to 80 ind/1) of mixed copepodids of Acartia tsuensis, Pseudodiaptomus spp., and Oithona sp. three days before stocking larvae. Rotifers were added in these tanks on Day 7 at an initial density of 5,000 ind/1. Larvae in the remaining tank were fed rotifers (only) starting Day 2 at 5,000 ind/l. The feeding incidence, gut content, growth, and survival of larvae were better in tanks with higher density of copepodids (60-80 ind/l). These indices were lowest in larvae given rotifers only. Total n-3 HUFA of copepods was 2 to 3 times higher than rotifers. High percentages of 22:6n-3 (DHA) were detected in the fatty acid composition of Pseudodiaptomus (13%) and Acartia (24%) with DHA/EPA (20:5n-3) values of 1.4 and 2.6, respectively. By providing nauplii of copepods at the early feeding stage, an average survival of 3.4% at harvest (Day 36) was obtained in a pilot scale grouper seed production trial in three 10-ton tanks.

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SUMMARY:The occurrence pattern and population dynamics of Acartia tsuensis were investigated in a brackish-water pond in Panay Island in central Philippines by implementing both bi-monthly and daily sampling schemes. A. tsuensis occurred in the pond during the dry season (November-April) when the salinity of the water is in the range of 14 40 ppt but was completely absent at lower salinities. An almost constant rate of development from the nauplius 2 through to copepodite 5 stages of A. tsuensis was observed both in the pond and in the laboratory. The generation time ranged from 5.9-11.3 days. Fecundity had a positive linear correlation with chlorophyll a. Salinity and chlorophyll a affect the stage duration, mortality, and fecundity of A. tsuensis in the pond.

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