Aptamer has been long studied as a substitute of antibodies for many purposes. However, due to the exceeded length of the aptamers obtained in vitro, difficulties arise in its manipulation during its molecular conjugation on the matrix surfaces. Current study focuses on computational improvement for aptamers screening of hepatitis B surface antigen (HBsAg) through optimization of the length sequences obtained from SELEX. Three original aptamers with affinity against HBsAg were truncated into five short hairpin structured aptamers and their affinity against HBsAg was thoroughly studied by molecular docking, molecular dynamics (MD) simulation, and Molecular Mechanics Poisson-Boltzmann Surface Area (MMPBSA) method. The result shows that truncated aptamers binding on HBsAg “a” determinant region are stabilized by the dynamic H-bond formation between the active binding residues and nucleotides. Amino acids residues with the highest hydrogen bonds hydrogen bond interactions with all five aptamers were determined as the active binding residues and further characterized. The computational prediction of complexes binding will include validations through experimental assays in future studies. Current study will improve the current in vitro aptamers by minimizing the aptamer length for its easy manipulation.

No abstract

Aptamers are oligonucleotides and peptides with short and medium length around-nucleotides or amino acids and a molecule of interests due to its specific binding affinities to the vast array of target molecules such as ions, complex proteins and antigens up to cellular surface and cell organelles. Aptamers shows high potential of application in therapeutics and diagnostics. Aptamers are usually obtained through rigorous in-vitro screening procedures known

No abstract

The appearance of Islam Nusantara has a long track record in the history. Twin kingdoms of Gowa-Tallo make Islam the "official religion" in 1605. Since then, the dialogue between the ideas of universal Islamic and cultural tradition-temporal locality Bugis-Makassar ongoing intense. Muslim villages in the District Village Tallo Tallo is a Muslim village that grew since 1605 with a population of 8,017 inhabitants and 100% are Muslim. Bugis-Islamic acculturation appears in architecture. Tallo communities still maintain the culture until now, the world-view of the Bugis-Makassar related Mappaenre culture 'balls or build a house that is a strong linkage between local traditions and Islamic values. Muslim settlement patterns in Tallo has a linear characteristic and spreads following the road network. Architecture Tallo Muslim villages and houses a cultural encounter Bugis-Makassar and Islam, especially in the building pillars, roof, doors, and windows.