The hairy root culture of halophyte was obtained by transforming seedlings' primary leaves with a wild strain of 15834 SWISS (transformation frequency was 78.7%). The roots' transgenic status was confirmed by PCR analysis using -,-, -,-specific primers. There was a significant increase in the biosynthesis of flavonoids, hydroxycinnamic acids, pectins, protopectins and saponins in hairy roots as compared to intact plant roots. The сatechin content in ethanol extracts of transformed roots' exceeded this factor 3.8 times in plant roots, but the compound composition of catechin nature in the latter was more diverse. The high antiviral activity of hairy root ethanol extracts against influenza virus subtypes A(H5N1), A(H3N2) was revealed, which manifested itself in the effective inhibition of infectivity both in vitroin MDCK cell culture-and in vivo increasing survival of mice infected with them.
The paper shows composition and content of phenolic compounds in leaves of 4 Nitraria L. species from 58 populations of Russia, Kazakhstan, and Tajikistan studied with high-performance liquid chromatography(HPLC). The investigation has revealed 27 compounds of phenolic nature: the maximum number (18 components) is detected in leaves of N. sibirica Pall. from Kazakhstan (the Karatal river valley), 16-17 components – in plants from three populations of Siberia, the minimum (6 components) – in leaves of N. komaroviiilljin & Lava ex Bobrov. The analysis has identified hyperoside (quercetin O-glycoside), narcissin (isoramnetin O-glycoside), quercetin (flavonol) and luteolin (flavon). The studied plants accumulate a generous quantity of phenolic compounds. Their content reaches 4.64% in leaves of N. sibirica, 3.11% – in N. schoberi L., up to 3.96% – in N. komarovii. The research results allow speaking about the species-specific composition and content of phenolic compounds of N. sibirica, N. schoberi and N. komarovii. The component composition is weaker in extracts of N. komarovii leaves, but there is a higher content of total phenolic compounds compared to N. schoberi plants. N. pamirica L. Vassil sample is close to N. schoberi on multidimensional analysis of the phenolic compound composition and content.
The research was carried out within the framework of project VI.52.1.2, "Analysis of the intraspecific structure of resource plants Version of Record 1127 TAXON 69 (5) • October 2020: 1126-1132 Marhold & Kuc ˇera (eds.) • IAPT chromosome data 32 in Asian Russia, selection and conservation of the gene pool" (No.
For the first time are search of nuclear DNA relative content in Nitraria schoberi L. seeds from 15 natural populations of Siberia, the Crimea, Kazakhstan, and Tajikistan has been conducted by the method of flow cytometry. High intra-and interpopulational variations of nuclear DNA content - 2,93-3,39 pg, at average value - 3,22±0,108 pg is revealed.
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