Nitraria schoberi L. hairy root culture as a source of compounds with antiviral activity against influenza virus subtypes А(H5N1) and А(H3N2)

Zheleznichenko, T. V.; Банаев, Е. В.; Asbaganov, Sergey; Voronkova, M. S.; Kukushkina, T. A.; Filippova, Ekaterina; Мазуркова, Н. А.; Шишкина, Л. Н.; Novikova, Tatyana

doi:10.1007/s13205-018-1280-5

Cited by 16 publications

(8 citation statements)

References 39 publications

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“…Chlorogenic acid derivatives were raised in HRs compared to NTRs of Stevia rebaudiana [32]. The content of flavonoids, hydroxycinnamic acids in the HRs was several times higher than their level in the intact plant roots of Nitraria schoberi [33]. In contrast, HRs and NTRs had a similar amount of phenolic compounds in Platycodon grandiflorum [34].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of Ligularia fischeri Turcz. f. spiciformis (Nakai)

et al. 2019

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A considerable amount of bioactive compounds have been used for the biopharmaceutical engineering to help human health and nutrition. Hairy root culture (HRC) or transgenic root is a favourable alternative technique for phytochemical production. Ligularia fischeri is a significant source of pharmaceutically important active compounds with an enormous range of health care applications. HRC of L. fischeri was developed using Agrobacterium rhizogenes for the production of polyphenolic compounds with antioxidant, antimicrobial, antidiabetic, anticancer and anti-inflammatory pharmaceutical activities. Hairy roots (HRs) were selected by morphological assessment, genetic and molecular analyses. The maximum accumulation of fresh mass (94.15 g/L) and dry mass (9.45 g/L) was recorded in MS liquid medium supplemented with 30 g/L sucrose at 28 days. Furthermore, HRs successfully produced numerous polyphenolic compounds, including six hydroxycinnamic acids, seven flavonols, seven hydroxybenzoic acids, vanillin, resveratrol, pyrogallol, homogentisic, and veratric acids, which were identified by UHPLC analysis. HRs produced higher total phenolic (185.65 mg/g), and flavonoid (5.25 mg/g) contents than non-transformed roots (125.55 mg/g and 3.75 mg/g). As a result of these metabolic changes, pharmaceutical activities were found higher in HRs than non-transformed roots (NTRs). The present study indicates that HRC has the potential to increase the content of beneficial polyphenolic compounds with higher potential pharmaceutical activities. To the best of our knowledge, the present study is the first report on enhancing the production of polyphenolic compounds with pharmaceutical activities from the HRCs of L. fischeri.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of Ligularia fischeri Turcz. f. spiciformis (Nakai)

et al. 2019

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“…Species of this genus are promising medicinal plants. It is known antioxidant [1,2], antigenotoxic [3], antiproliferative [4], cytotoxic [5,6,7,8], antimicrobial, antiviral [9], antimutagenic, hypotensive [10], cardioprotective [11], hypolipidemic, hypoglycemic [1], hepatoprotective properties of these plant extracts [12] depending on a complex of biologically active substances in them. Secondary metabolites of the Nitraria L. genus plants are mainly represented by flavonoids and alkaloids, but the most researches are devoted to investigating alkaloids [12].…”

Section: Introductionmentioning

confidence: 99%

Possibilities of using the HPLC method in the taxonomy of the genus Nitraria (Nitrariaceae)

et al. 2020

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The paper shows composition and content of phenolic compounds in leaves of 4 Nitraria L. species from 58 populations of Russia, Kazakhstan, and Tajikistan studied with high-performance liquid chromatography(HPLC). The investigation has revealed 27 compounds of phenolic nature: the maximum number (18 components) is detected in leaves of N. sibirica Pall. from Kazakhstan (the Karatal river valley), 16-17 components – in plants from three populations of Siberia, the minimum (6 components) – in leaves of N. komaroviiilljin & Lava ex Bobrov. The analysis has identified hyperoside (quercetin O-glycoside), narcissin (isoramnetin O-glycoside), quercetin (flavonol) and luteolin (flavon). The studied plants accumulate a generous quantity of phenolic compounds. Their content reaches 4.64% in leaves of N. sibirica, 3.11% – in N. schoberi L., up to 3.96% – in N. komarovii. The research results allow speaking about the species-specific composition and content of phenolic compounds of N. sibirica, N. schoberi and N. komarovii. The component composition is weaker in extracts of N. komarovii leaves, but there is a higher content of total phenolic compounds compared to N. schoberi plants. N. pamirica L. Vassil sample is close to N. schoberi on multidimensional analysis of the phenolic compound composition and content.

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“…The growth productivity of in vitro callus, suspension cells and root cultures, depending on the strain of Agrobacterium and the temperature of cultivation, is presented in Table 2 [ 68 ].…”

Section: The Use Of Agrobacteria In the In Vitro Production Of Calmentioning

confidence: 99%

“…Strains A5, A7, TR107, LBA920, LBA9435, AR25, A4PC, LMG63, AM8703, A2/83, A20/83, AR1193, NIAES1724 and MTCC2364, were used in two research studies each. A very large number of analyzed strains were used by the authors only once [ 68 ].…”

Section: The Use Of Agrobacteria In the In Vitro Production Of Calmentioning

confidence: 99%

Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants

et al. 2020

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This paper studies modern methods of producing and using callus, suspension cells and root cultures of medicinal plants in vitro. A new solution for natural product production is the use of an alternative source of renewable, environmentally friendly raw materials: callus, suspension and root cultures of higher plants in vitro. The possibility of using hairy root cultures as producers of various biologically active substances is studied. It is proven that the application of the genetic engineering achievements that combine in vitro tissue culture and molecular biology methods was groundbreaking in terms of the intensification of the extraction process of compounds significant for the medical industry. It is established that of all the callus processing methods, suspension and root cultures in vitro, the Agrobacterium method is the most widely used in practice. The use of agrobacteria has advantages over the biolistic method since it increases the proportion of stable transformation events, can deliver large DNA segments and does not require special ballistic devices. As a result of the research, the most effective strains of agrobacteria are identified.

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Nitraria schoberi L. hairy root culture as a source of compounds with antiviral activity against influenza virus subtypes А(H5N1) and А(H3N2)

Cited by 16 publications

References 39 publications

Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of Ligularia fischeri Turcz. f. spiciformis (Nakai)

Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of Ligularia fischeri Turcz. f. spiciformis (Nakai)

Possibilities of using the HPLC method in the taxonomy of the genus Nitraria (Nitrariaceae)

Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants

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