The use of microbore liquid chromatography columns for high-performance liquid chromatography/mass spectrometry provides improved sensitivity for interfaces of the direct liquid introduction and moving belt types. In the latter case the use of the technique also assists in the handling of mobile phases containing a high percentage of water. Methods for improving the efficiency of microbore columns made from PTFE are described together with the use of a manual column switching technique to assist in some of the problems encountered due to column overload with microbore liquid chromatography columns. The utility of microbore liquid chromatography/mass spectrometry is illustrated by the analysis of an aqueous solution of phenolic compounds.
Combined liquid chromatographylmass spectrometry using a moving belt interface and commercially available glass lined stainless steel microbore reverse phase liquid chromatographic columns is described. The chromatographic performance is comparable with that obtained with conventional columns, and the lower mobile phase flow rates used enable improved liquid chromatographic/mass spectrometric sensitivities to be obtained since all the liquid chromatographic eluent can be handled by the interface and instrumental background due to solvent impurities is reduced. The utility of the approach is illustrated with studies of extracts from timbo powder, Zmperatoria oshurhium and Culophyllurn inophyllum. Using the carbamate pesticides IPC and CIPC the potential of microbore liquid chromatographylmass spectrometry for quantitative studies is demonstrated.
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