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We present a high resolution, ultra-frugal printing of paper microfluidic
devices using in-house paraffin formulation on a simple filter paper. The patterns
printed using an office inkjet printer formed a selective hydrophobic barrier of
4 ± 1 µm thickness with a hydrophilic channel width of 275 µm. These printed patterns
effectively confine common aqueous solutions and solvents, which was verified by solvent
compatibility studies. SEM analysis reveals that the solvent confinement is due to pore
blockage in the filter paper. The fabricated paper-based device was validated for
qualitative assessment of Candida albicans
(pathogenic fungi) by using a combination of L-proline β-naphthylamide as the substrate
and cinnamaldehyde as an indicator. Our studies reveal that the pathogenic fungi can be
detected within 10 min with the limit of detection (LOD) of
0.86 × 106 cfu/mL. Owing to its simplicity, this facile
method shows high potential and can be scaled up for developing robust paper-based
devices for biomarker detection in resource-limited settings.
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